Antibody immobilized cysteamine functionalized-gold nanoparticles for aflatoxin detection
IR@NPL: CSIR-National Physical Laboratory, New Delhi
View Archive Info| Field | Value | |
| Title |
Antibody immobilized cysteamine functionalized-gold nanoparticles for aflatoxin detection
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| Creator |
Sharma, Aditya
Matharu, Zimple Sumana, G. Solanki, Pratima R. Kim, C. G. Malhotra, B. D. |
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| Subject |
Materials Science
Physics |
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| Description |
Aflatoxin B1 antibody (aAFB1) covalently attached to cysteamine functionalized-gold nanoparticles (C-AuNP) has been immobilized onto 4-mercaptobenzoic acid (MBA) based self assembled monolayer (SAM) on gold electrode (MBA/Au), for the fabrication of BSA/aAFB1-C-AuNP/MBA/Au immunoelectrode. This immunoelectrode has been characterized by Fourier Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM) and electrochemical characterization techniques. The electrochemical response studies reveal that the BSA/aAFB1-C-AuNP/MBA/Au immunoelectrode can be used to detect AFB1 in the range of 10–100 ng dL− 1 and has sensitivity as 0.45 μA ng− 1 dL, limit of detection as 17.90 ng dL− 1 and a response time of 60 s.
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| Publisher |
Elsevier
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| Date |
2010-08-25
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://npl.csircentral.net/25/1/16.pdf
Sharma, Aditya and Matharu, Zimple and Sumana, G. and Solanki, Pratima R. and Kim, C. G. and Malhotra, B. D. (2010) Antibody immobilized cysteamine functionalized-gold nanoparticles for aflatoxin detection. Thin Solid Films, 519 (3). pp. 1213-1218. ISSN 0040-6090 |
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| Relation |
http://www.sciencedirect.com/science/article/pii/S0040609010011867
http://npl.csircentral.net/25/ |
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