Application of nanostructured ZnO films for electrochemical DNA biosensor
IR@NPL: CSIR-National Physical Laboratory, New Delhi
View Archive Info| Field | Value | |
| Title |
Application of nanostructured ZnO films for electrochemical DNA biosensor
|
|
| Creator |
Das, Maumita
Sumana, Gajjala R. , Nagarajan Malhotra, B. D. |
|
| Subject |
Materials Science
Physics |
|
| Description |
Nanostructured zinc oxide (nsZnO) films have been fabricated onto conducting indium–tin–oxide (ITO) coated glass plate, by cathodic electro-deposition to immobilize probe DNA specific to M. tuberculosis via physisorption based on strong electrostatic interactions between positively charged ZnO (isoelectric point = 9.5) and negatively charged DNA to detect its complementary target. Electrochemical studies reveal that the presence of nano-structured ZnO results in increased electro-active surface area for loading of DNA molecules. The DNA–nsZnO/ITO bioelectrode exhibits interesting characteristics such as detection range of 1 × 10-6 - 1 × 10-12 M, detection limit of 1 × 10-12 M (complementary target) and 1 × 10-13 M (genomic DNA), reusability of about 10 times, response time of 60s and stability of up to 4 months when kept at 4°C.
|
|
| Publisher |
Elsevier
|
|
| Date |
2010-08-20
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://npl.csircentral.net/26/1/17.pdf
Das, Maumita and Sumana, Gajjala and R. , Nagarajan and Malhotra, B. D. (2010) Application of nanostructured ZnO films for electrochemical DNA biosensor. Thin Solid Films, 519 (3). pp. 1196-1201. ISSN 0040-6090 |
|
| Relation |
http://www.sciencedirect.com/science/article/pii/S0040609010011843
http://npl.csircentral.net/26/ |
|