Fast seperation and sensitive detection of carcinogenic aromatic amines by reversed phase liquid chromatography coupled with electrochemical detection.
IR@AMPRI: CSIR-Advanced Materials and Processes Research Institute, Bhopal
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| Title |
Fast seperation and sensitive detection of carcinogenic aromatic amines by reversed phase liquid chromatography coupled with electrochemical detection.
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| Creator |
Manjusha, Shelke
S.K., Sanghi Amit, Asthana Sushma, Lamba Meenakshi , Sharma |
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| Subject |
Environmental Studies/Chemistry
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| Description |
A micro-LC method was developed for the fast and sensitive analysis of aromatic amines by electrochemical detection. The chromatographic separation of nine carcinogenic aromatic amines was performed on an ABZ + PLUS column with detection limits up to pM L(-1) levels. Mobile phase comprised of methanol-acetate buffer of pH 5 (45:55, v/v) used at a flow rate of 0.2 ml min(-1). The detection was performed with a 6 mm glassy carbon electrode at an applied potential of 0.8 V versus Ag/AgCl. An intraday RSD for retention time and peak area were between 0.22% and 0.73% and 1.86% and 4.03%, respectively. The interdays RSD for retention time and peak area were between 0.47% and 1.35% and 2.04% and 4.42%, respectively. The applicability of the assay has been demonstrated by analyzing these aromatic amines in lake water and synthetic food colour additives. A comparison is given between electrochemical and UV detection.
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ELSEVIER
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| Date |
2005
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| Type |
Article
PeerReviewed |
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| Identifier |
Manjusha, Shelke and S.K., Sanghi and Amit, Asthana and Sushma, Lamba and Meenakshi , Sharma (2005) Fast seperation and sensitive detection of carcinogenic aromatic amines by reversed phase liquid chromatography coupled with electrochemical detection. Journal of chromatography A, 1089. pp. 52-58.
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| Relation |
http://ampri.csircentral.net/622/
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