Immobilization of monoamine oxidase on eggshell membrane and its application in designing an amperometric biosensor for dopamine.
IR@AMPRI: CSIR-Advanced Materials and Processes Research Institute, Bhopal
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Title |
Immobilization of monoamine oxidase on eggshell membrane and its application in designing an amperometric biosensor for dopamine.
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Creator |
P., Joshi
H. C. , Joshi, S. K. , Sanghi S., Kundu |
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Subject |
Environmental Studies/Chemistry
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Description |
An amperometric biosensor for dopamine is described. It is based on the enzyme monoamine oxidase immobilized on a glutaraldehyde-activated eggshell membrane that was deposited on a glassy carbon electrode. The Michaelis-Menten constant (Km) is 0.087 mM. Optimum pH and temperature conditions were obtained at pH 7.0 and 37 °C, respectively. The sensor showed a detection limit of 20 μM, a linear range from 50 μM to 250 μM, and a storage stability of ~25 days. In order to further improve the performance, a Nafion coating was applied on the electrode surface which gave favorable results with respect to shelf life of the enzyme (~40 days), the limit of detection, and the selectivity over ascorbic acid and uric acid.
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Publisher |
Springer Vienna
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Date |
2010
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Type |
Article
PeerReviewed |
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Format |
application/pdf
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Identifier |
http://ampri.csircentral.net/810/1/10.1007%2Ds00604%2D010%2D0346%2D9.pdf
P., Joshi and H. C. , Joshi, and S. K. , Sanghi and S., Kundu (2010) Immobilization of monoamine oxidase on eggshell membrane and its application in designing an amperometric biosensor for dopamine. Microchimica Acta, 169. pp. 383-388. ISSN 0026-3672 |
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Relation |
http://ampri.csircentral.net/810/
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