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Osteogenic activity of natural diterpenoids in calvarial derived osteoblast cells via differentiation and mineralization

IR@CDRI: CSIR-Central Drug Research Institute, Lucknow

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Field Value
 
Creator Khan, M F
Dev, Kapil
Lahiri, Shibani
Dixit, Manisha
Trivedi, Ritu
Singh, Divya
Maurya, Rakesh
 
Date 2015-05-13T06:02:33Z
2015-05-13T06:02:33Z
2014
 
Identifier Phytomedicine, 2014, 21(14), 1794-800
http://hdl.handle.net/123456789/1449
 
Description The aim of the present study was to investigate the antiosteoporotic activity of four structurally related diterpenoids: sugiol (1), trans-communic acid (2), 15-acetoxy imbricatolic acid (3) and imbricatolic acid (4). Their osteogenic effect was evaluated by using validated models including alkaline phosphatase (ALP) assay, mineralization assay and expression of osteogenic genes-bone morphogenetic protein-2(BMP2) and osteoblast transcription factor (RUNX2) – in primary calvarial cultures harvested from neonatal mice. Among them, compound 1 at a dose of 1.0 mg/kg body weight exhibited significant osteoprotective effects and did not show uterine estrogenicity at the same dose. Additionally, compound 1 treatment led to improved biomechanical properties as exhibited by increased power, energy and stiffness in femoral bones compared to untreated Ovx animals. Since osteoporotic compression fracture correlates with the mechanical characteristics of trabecular bone, so that it could effectively reduce the risk of this type of fracture by improving trabecular micro architecture in postmenopausal women. Therefore, our findings proposed that diterpenoids may be useful new chemical agents in the treatment of diseases associated with bone lose
 
Format 801333 bytes
application/pdf
 
Language en
 
Relation CSIR-CDRI Communication No. 8804
 
Subject Alkaline phosphatase
Cupressus sempervirens
Diterpenoids
Osteoblast differentiation
Osteoporosis
Sugiol
 
Title Osteogenic activity of natural diterpenoids in calvarial derived osteoblast cells via differentiation and mineralization
 
Type Article