Conversion of Amyloid Fibrils of Cytochrome c to Mature Nanorods through a Honeycomb Morphology
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Conversion of Amyloid Fibrils of Cytochrome c to Mature Nanorods through a Honeycomb Morphology
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| Creator |
Haldar, Shubhasis
Sil, Pallabi Thangamuniyandi, Muruganandan Chattopadhyay, Krishnananda |
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| Subject |
Structural Biology & Bioinformatics
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| Description |
ABSTRACT: Amyloid species with various morphologies have been found for different proteins and disease systems. In this article, we aim to ask if these morphologies are
unique to a particular protein or if they convert from one to another. Using a heme protein containing iron as the transition-metal activator of aggregation and a negatively
charged surfactant, partial unfolding of the protein and its aggregation have been induced. In the pathway of aggregation, we have observed the formation of several
morphological structures of a single protein, which were visualized directly using atomic force microscopy (AFM). These structures have been found to appear and disappear
with time, and their formation could be monitored under normal buffer conditions and at room temperature without requiring any sophisticated chemical or biological
methodologies. In addition, we have observed the formation of honeycomb-shaped morphology, which may serve as an intermediate. These amyloid-based nanostructures
may have the potential to be explored in therapeutics delivery and other biomedical applications.
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| Publisher |
American Chemical Society
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| Date |
2015
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/2237/1/LANGMUIR__V._31__(_14_)4213%2D4223;2015[29].pdf
Haldar, Shubhasis and Sil, Pallabi and Thangamuniyandi, Muruganandan and Chattopadhyay, Krishnananda (2015) Conversion of Amyloid Fibrils of Cytochrome c to Mature Nanorods through a Honeycomb Morphology. Langmuir, 31 (14). pp. 4212-4223. |
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| Relation |
http://dx.doi.org/10.1021/la5029993
http://www.eprints.iicb.res.in/2237/ |
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