TALEN mediated targeted editing of GM2/GD2-synthase gene modulates anchorage independent growth by reducing anoikis resistance in mouse tumor cells
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
TALEN mediated targeted editing of GM2/GD2-synthase gene modulates anchorage independent growth by reducing anoikis resistance in mouse
tumor cells
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| Creator |
Mahata, Barun
Banerjee, Avisek Kundu, Manjari Bandyopadhyay, Uday Biswas, Kaushik |
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| Subject |
Infectious Diseases and Immunology
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| Description |
Complex ganglioside expression is highly deregulated in several tumors which is further dependent on
specific ganglioside synthase genes. Here, we designed and constructed a pair of highly specific
transcription-activator like effector endonuclease (TALENs) to disrupt a particular genomic locus of mouse
GM2-synthase, a region conserved in coding sequence of all four transcript variants of mouse
GM2-synthase. Our designed TALENs effectively work in different mouse cell lines and TALEN induced
mutation rate is over 45%. Clonal selection strategy is undertaken to generate stable GM2-synthase
knockout cell line. We have also demonstrated non-homologous end joining (NHEJ) mediated integration
of neomycin cassette into the TALEN targeted GM2-synthase locus. Functionally, clonally selected
GM2-synthase knockout clones show reduced anchorage-independent growth (AIG), reduction in tumor
growth and higher cellular adhesion as compared to wild type Renca-v cells. Insight into the mechanism
shows that, reduced AIG is due to loss in anoikis resistance, as both knockout clones show increased
sensitivity to detachment induced apoptosis. Therefore, TALEN mediated precise genome editing at
GM2-synthase locus not only helps us in understanding the function of GM2-synthase gene and complex
gangliosides in tumorigenicity but also holds tremendous potential to use TALENs in translational cancer
research and therapeutics.
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| Date |
2015
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/2252/1/SCIENTIFIC_REPORTS__V._5_____Article_Number_9048.pdf
Mahata, Barun and Banerjee, Avisek and Kundu, Manjari and Bandyopadhyay, Uday and Biswas, Kaushik (2015) TALEN mediated targeted editing of GM2/GD2-synthase gene modulates anchorage independent growth by reducing anoikis resistance in mouse tumor cells. Scientific Reports, 5 (AN9048). |
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| Relation |
http://dx.doi.org/10.1038/srep09048
http://www.eprints.iicb.res.in/2252/ |
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