mRNA Targeting to Endoplasmic Reticulum Precedes Ago Protein Interaction and MicroRNA (miRNA)-mediated Translation Repression in Mammalian Cells
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
mRNA Targeting to Endoplasmic Reticulum Precedes Ago Protein
Interaction and MicroRNA (miRNA)-mediated Translation
Repression in Mammalian Cells
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| Creator |
Barman, Bahnisikha
Bhattacharyya, Suvendra Nath |
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| Subject |
Molecular & Human Genetics
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| Description |
MicroRNA (miRNA) binds to the 3�-UTR of its target mRNAs
to repress protein synthesis. Extensive research was done to
understand the mechanism of miRNA-mediated repression in
animal cells. Considering the progress in understanding the
mechanism, information about the subcellular sites of
miRNA-mediated repression is surprisingly limited. In this
study, using an inducible expression system for an miRNA
target message, we have delineated how a targetmRNApasses
through polysome association and Ago2 interaction steps on
rough endoplasmic reticulum (ER) before the miRNA-mediated
repression sets in. From this study, de novo formed target
mRNA localization to the ER-bound polysomes manifested as
the earliest event, which is followed by Ago2 micro-ribonucleoprotein binding, and translation repression of target message. Compartmentalization of this process to rough ER membrane ensures enrichment of miRNA-targeted messages and micro-ribonucleoprotein components on ER upon reaching a steady state
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| Publisher |
American Society for Biochemistry and Molecular Biology
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| Date |
2015
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/2337/1/JOURNAL_OF_BIOLOGICAL_CHEMISTRY__V__290(__41_)_24650%2D24656_;2015;[4].pdf
Barman, Bahnisikha and Bhattacharyya, Suvendra Nath (2015) mRNA Targeting to Endoplasmic Reticulum Precedes Ago Protein Interaction and MicroRNA (miRNA)-mediated Translation Repression in Mammalian Cells. The Journal of Biological Chemistry, 290 (41). pp. 24650-25656. |
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| Relation |
http://dx.doi.org/10.1074/jbc.C115.661868
http://www.eprints.iicb.res.in/2337/ |
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