Bioremediation by alkaline protease (AkP) from edible mushroom Termitomyces clypeatus: optimization approach based on statistical design and characterization for diverse applications
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Bioremediation by alkaline protease (AkP) from edible mushroom Termitomyces clypeatus: optimization approach based on statistical design and characterization for diverse
applications
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| Creator |
Majumder, Rajib
Banik, Samudra Prosad Ramrakhiani, Lata Khowala, Suman |
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| Subject |
Drug Development/Diagnostics & Biotechnology
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| Description |
BACKGROUND: Bioremediation using enzymes has become an attractive approach for removing hazardous chemicals from the environment. The present study investigated theproduction, optimization andapplications of an extracellular alkalineprotease(AkP) fromthe edible mushroom Termitomyces clypeatus focusing on bioremediation.
RESULTS: For optimized enzyme production, the variables significantly influencing the protease activitywere screened through the 11 factorial Plackett–Burman design. The optimum values of the selected variables and their mutual interactions were determined through response surfacemethodology using the Box–Behnken experimental design. Overall a 10-fold increase in enzyme activity (1830 UmL-1)was obtained which closely matched the yield predicted by the statisticalmodel (1778.63 UmL-1)
with R2 =0.9819. AkP could efficiently dehair the goat skin, detached bird feather vanes from its shaft and reduce BOD, COD and pH of tannery waste effluent. The AkP also demonstrated bactericidal property against Staphylococcus aureus as detected by MIC and flow cytometry analyses.
CONCLUSION: As an ecofriendly alternative, the enzyme showed significant promise for bioremediation and industrial applications through time-saving bioprocesses. This is first report of alkaline protease from T. clypeatus or from a fungal source with wide-ranging application potential.
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| Publisher |
John Wiley & Sons
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| Date |
2015
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/2356/1/Majumder_et_al%2D2015%2DJournal_of_Chemical_Technology_and_Biotechnology_(5).pdf
Majumder, Rajib and Banik, Samudra Prosad and Ramrakhiani, Lata and Khowala, Suman (2015) Bioremediation by alkaline protease (AkP) from edible mushroom Termitomyces clypeatus: optimization approach based on statistical design and characterization for diverse applications. Journal of Chemical Technology & Biotechnology, 90 (10). pp. 1886-1896. ISSN 0268-2575 |
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| Relation |
http://dx.doi.org/10.1002/jctb.4500
http://www.eprints.iicb.res.in/2356/ |
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