Cancer Cell Imaging Using in Situ Generated Gold Nanoclusters
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Cancer Cell Imaging Using in Situ Generated Gold
Nanoclusters
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| Creator |
Chattoraj, Shyamtanu
Amin, Md. Asif Mohapatra, Saswat Ghosh, Surajit Bhattacharyya, Kankan |
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| Subject |
Chemistry
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| Description |
In situ generated fluorescent gold nanoclusters (Au-NCs) are
used for bio-imaging of three human cancer cells, namely, lung (A549), breast (MCF7), and colon (HCT116), by confocal microscopy. The amount of Au-NCs in non-cancer cells (WI38 and MCF10A) is 20–40 times less than those in the corresponding cancer cells. The presence of a larger amount of glutathione (GSH) capped Au-NCs in the cancer cell is ascribed to a higher glutathione level in cancer cells. The Au-NCs exhibit fluorescence maxima at 490–530 nm inside the cancer cells. The fluorescence maxima and matrix-assisted laser desorption ionization (MALDI) mass spectrometry suggest that the fluorescent Au-NCs consist of GSH capped clusters with a core structure (Au8-13). Time-resolved confocal microscopy indicates a nanosecond (1–3 ns) lifetime of the Au-NCs inside the cells. This rules
out the formation of aggregated Au–thiolate complexes,
which typically exhibit microsecond (�1000 ns) lifetimes. Fluorescence correlation spectroscopy (FCS) in live cells indicates that the size of the Au-NCs is �1–2 nm. For in situ generation,we used a conjugate consisting of a room-temperature ionic liquid (RTIL, [pmim][Br]) and HAuCl4. Cytotoxicity studies indicate that the conjugate, [pmim][AuCl4], is non-toxic for both cancer and non-cancer cells.
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| Publisher |
Wiley-VCH Verlag Berlin
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| Date |
2016
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/2447/1/CHEMPHYSCHEM__Vol._17(__1__)61%2D68;2016.pdf
Chattoraj, Shyamtanu and Amin, Md. Asif and Mohapatra, Saswat and Ghosh, Surajit and Bhattacharyya, Kankan (2016) Cancer Cell Imaging Using in Situ Generated Gold Nanoclusters. ChemPhysChem, 17 (1). pp. 61-68. |
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| Relation |
http://dx.doi.org/10.1002/cphc.201500731
http://www.eprints.iicb.res.in/2447/ |
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