E. coli metabolic protein aldehydealcohol dehydrogenase-E binds to the ribosome: a unique moonlighting action revealed
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
E. coli metabolic protein aldehydealcohol dehydrogenase-E binds to the ribosome: a unique moonlighting action revealed
|
|
| Creator |
Shasmal, Manidip
Dey, Sandip Shaikh, Tanvir R Bhakta, Sayan Sengupta, Jayati |
|
| Subject |
Structural Biology & Bioinformatics
|
|
| Description |
It is becoming increasingly evident that a high degree of regulation is involved in the protein synthesis machinery entailing more interacting regulatory factors. A multitude of proteins have been identified recently which show regulatory function upon binding to the ribosome. Here, we identify tight association of a metabolic protein aldehyde-alcohol dehydrogenase E (AdhE) with the E. coli 70S
ribosome isolated from cell extract under low salt wash conditions. Cryo-EM reconstruction of the ribosome sample allows us to localize its position on the head of the small subunit, near the mRNA entrance. Our study demonstrates substantial RNA unwinding activity of AdhE which can account for the ability of ribosome to translate through downstream of at least certain mRNA helices. Thus far, in
E. coli, no ribosome-associated factor has been identified that shows downstream mRNA helicase activity. Additionally, the cryo-EM map reveals interaction of another extracellular protein, outer membrane protein C (OmpC), with the ribosome at the peripheral solvent side of the 50S subunit. Our result also provides important insight into plausible functional role of OmpC upon ribosome binding.
Visualization of the ribosome purified directly from the cell lysate unveils for the first time interactions
of additional regulatory proteins with the ribosome
|
|
| Date |
2016
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://www.eprints.iicb.res.in/2458/1/SCIENTIFIC_REPORTS__Vol.__6___Article_Number__19936;2016.pdf
Shasmal, Manidip and Dey, Sandip and Shaikh, Tanvir R and Bhakta, Sayan and Sengupta, Jayati (2016) E. coli metabolic protein aldehydealcohol dehydrogenase-E binds to the ribosome: a unique moonlighting action revealed. Scientific Report, 6 (19936). 01-12. |
|
| Relation |
http://dx.doi.org/10.1038/srep19936
http://www.eprints.iicb.res.in/2458/ |
|