Structural and functional characterization of type three secretion associated proteins from Yersinia enterocolitica and vitamin biosynthesis pathway proteins from Pseudomonas aeruginosa
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Structural and functional characterization of type three secretion associated proteins from Yersinia enterocolitica and vitamin biosynthesis pathway proteins from Pseudomonas aeruginosa
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| Creator |
Chatterjee, Rakesh
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| Subject |
Structural Biology & Bioinformatics
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| Description |
Numerous gram negative bacteria employ contact dependent Type Three Secretion System
(T3SS) to inject virulence proteins into their host. Species like Yersinia, Pseudomonas, Shigella,
Xanthomonas, Bordetella, Erwinia, Escherechia coli can infect human, animals and plants causing a
number of diseases. T3SS uses a multiprotein complex structure (‘injectisome’) embedded into bacterial
cell to inject toxins which targets different signaling cascade in their respective hosts. T3SS consists of
different types of proteins : apparatus proteins- involved in architecture of injectisome; translocatorssits
on top of the injectisome to create a protein into host cell membrane; effectors-virulence toxins
chaperones specific for effectors/translocators and proteins involved in regulation, i.e. regulators.
Yersinia enterocolitica is an enteropathogenic bacteria which causes gastrointestinal infection
including ‘yersiniosis'. In immunocompromised adults and children, systemic infection by this
bacteria leads to high mortality rates. In addition to the plasmid encoded Ysc-yop T3SS, a
genomic encoded 200kb YSA-PI region T3SS also exists in Yersinia enterocolitica, called ysaysp
system. Apart from this region there are also some T3SS genes scattered across the geno of Yersinia enterocolitica. YsaN is a putative ATPase in this genomic encoded T3SS as inferred from sequence homology. Pseudomonas aeruginosa is an opportunistic pathogen associated with diseases like pneumonia, urinary tract infection, nosocomial infections etc in immunocompromised individuals, pateients with burns and wounds. So far very little medication is available to kill this organism. Microorganism and plants are capable of producing their own vitamins using specific pathways catalysed by certain enzymes. However humans usually depend on dietary source to assimilate vitamins. Hence these unique pathways can be used as potential drug targets for complete eradication of such organism. Pantothenate and Coenzyme A biosynthesis pathway is a significant pathway in this regard. Pantothenate is an important component of CoA required in central metabolism like fatty acid synthesis as well as in synthesis of polyketides. CoA is synthesized from pantothenate in five steps and the penultimate step is catalyzed by phosphopantethiene adenylyltransferase (PPAT), a product of coaD gene.
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| Date |
2016
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| Type |
Thesis
NonPeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/2575/1/Rakesh_Chatterjee_Thesis_._pdf.pdf
Chatterjee, Rakesh (2016) Structural and functional characterization of type three secretion associated proteins from Yersinia enterocolitica and vitamin biosynthesis pathway proteins from Pseudomonas aeruginosa. PhD thesis, C U. |
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| Relation |
http://www.eprints.iicb.res.in/2575/
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