Temperature-Induced Stress on Growth and Expression of Hsp in Freshwater alga Scenedesmus quadricauda1
IR@NEERI: National Environment Engineering Research Institute
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| Title |
Temperature-Induced Stress on Growth and Expression of Hsp in Freshwater alga Scenedesmus quadricauda1
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| Creator |
Zargar, S
Krishnamurthi, K Saravana Devi, S Ghosh, T K Chakrabarti, T |
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| Subject |
Environmental Biotechnology
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| Description |
Objective To investigate the impact of various levels of sublethal temperature (26'C, 31'C, 33'C, 36'C, and 39'C) on
growth and heat shock protein (hsp) expression in freshwater green alga Scenedesmus quadricauda. Methods Impact of selected levels of temperature on growth rate (based on optical density), population count, chlorophyll-a and biomass of the alga was evaluated in artificial growth medium for 19 days. To determine the induction of hsp in the alga, it was exposed to selected temperature levels for 3 h and further kept for 6 h at culturing condition at 26'C. Induction of hsp was confirmed by imrnuno-detection followed by SDS-polyacrylamide gel electrophoresis. Results The selected growth parameters such as growth rate, population count, chlorophyll-a and biomass were reduced significantly (P<O.OOl) at 39'C. However, hsp 70 expression was observed only at 39'C. Conclusion Temperature up to 36'C may be considered as the limit of safe exposure for thermal stress for the alga Scenedesmus quadricauda.
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| Date |
2006
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://neeri.csircentral.net/65/1/Zargar1.pdf
Zargar, S and Krishnamurthi, K and Saravana Devi, S and Ghosh, T K and Chakrabarti, T (2006) Temperature-Induced Stress on Growth and Expression of Hsp in Freshwater alga Scenedesmus quadricauda1. BIOMEDICAL AND ENVIRONMENTAL SCIENCES, 19. pp. 414-421. |
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| Relation |
http://neeri.csircentral.net/65/
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