Identification of over expressed proteins in oral submucous fibrosis by proteomic analysis
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Identification of over expressed proteins in oral submucous
fibrosis by proteomic analysis
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| Creator |
Das, Tapasi
Prodhan, Chandraday Patsa, Santanu Ray, Jay Gopal Chaudhuri, Keya |
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| Subject |
Molecular & Human Genetics
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| Description |
Early detection and identification of oral pre-malignancy or malignancy help in management of the disease and improve survival rates. Oral submucous fibrosis (OSMF)
is amajor threat to public health worldwide and especially in SoutheastAsian countries.Identification of biomarkers is a necessary step toward early diagnosis and treatment. In
this study, differentially expressed proteins between oral submucous fibrotic tissue and normal control tissues were recorded by proteomic analysis using two dimensional
electrophoresis (2DE) andMALDI TOF mass spectrometry. By proteomic analysis, 15 proteins were found to be upregulated and 10 proteins downregulated in the OSMF
tissues than the control tissues; among these identified proteins, Hsp-70 1B,Calreticulin, and Lumican variant exhibited higher expression in OSMF tissues compared to the
control tissues. Immunohistochemical analysis also showed elevated expression of these in OSMF tissues. Further validation was done by real time quantitative RT-PCR
analysis; gene expression of Hsp-70 1B, Calreticulin, and Lumican variant were significantly increased (6.2-, 3.3-, 2.8- fold, respectively), whereas Enolase 1 was
decreased by 0.5 fold in the OSMF tissues, consistent with proteomic results. The expression of proteins indicates that various cellular signaling pathways must be
involved in the processes of fibrosis and suggests that expressed protein molecules play an important role in the pathogenesis of OSMF. These identified proteins may be
potentially used in future studies of OSMF enabling to determine diagnostic marker or therapeutic targets of this precancerous condition of oral cavity
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| Publisher |
John Wiley & Sons
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| Date |
2018
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/2757/1/Jl._of_cellular_biochemistry.pdf
Das, Tapasi and Prodhan, Chandraday and Patsa, Santanu and Ray, Jay Gopal and Chaudhuri, Keya (2018) Identification of over expressed proteins in oral submucous fibrosis by proteomic analysis. Journal of Cellular Biochemistry, 119. pp. 4361-4371. ISSN 0730-2312 |
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| Relation |
http://dx.org.doi. 10.1002/jcb.26423
http://www.eprints.iicb.res.in/2757/ |
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