Aptamer based electrochemical sensor for detection of human lung adenocarcinoma A549 cells
IR@NPL: CSIR-National Physical Laboratory, New Delhi
View Archive Info| Field | Value | |
| Title |
Aptamer based electrochemical sensor for detection of human lung adenocarcinoma A549 cells
|
|
| Creator |
Sharma, Rachna
Agrawal, Ved Varun Sharma, Pradeep Varshney, R. Sinha, R. K. Malhotra, B. D. |
|
| Subject |
Applied Physics/Condensed Matter
Nanoscience/ Nanotechnology |
|
| Description |
We report results of the studies relating to development of an aptamer-based electrochemical biosensor for detection of human lung adenocarcinoma A549 cells. The aminated 85-mer DNA aptamer probe specific for the A549 cells has been covalently immobilized onto silane self assembled monolayer (SAM) onto ITO surface using glutaraldehyde as the crosslinker. The results of cyclic voltammetry and differential pulse voltammetry studies reveal that the aptamer functionalized bioelectrode can specifically detect lung cancer cells in the concentration range of 10(3) to 10(7) cells/ml with detection limit of 10(3) cells/ml within 60 s. The specificity studies of the bioelectrode have been carried out with control KB cells. No significant change in response is observed for control KB cells as compared to that of the A549 target cells.
|
|
| Publisher |
IOP Publishing
|
|
| Date |
2012
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://npl.csircentral.net/3689/1/Aptamer%20based%20electrochemical%20sensor%20for%20detection.pdf
Sharma, Rachna and Agrawal, Ved Varun and Sharma, Pradeep and Varshney, R. and Sinha, R. K. and Malhotra, B. D. (2012) Aptamer based electrochemical sensor for detection of human lung adenocarcinoma A549 cells. Journal of Physics: Conference Series , 358. 012001-012009. ISSN 1742-6588 |
|
| Relation |
http://npl.csircentral.net/3689/
|
|