Development and Validation of RP-HPLC Method for Estimation of Methotrexate Drug Intercalated in Mg-Al Layered Double Hydroxide Nanoparticles
IR@CGCRI: CSIR-Central Glass and Ceramic Research Institute, Kolkata
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| Title |
Development and Validation of RP-HPLC Method for Estimation of Methotrexate Drug Intercalated in Mg-Al Layered Double Hydroxide Nanoparticles
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| Creator |
Basu, Arindam
Ray, Sayantan Saha, Suman Chakraborty, Jui |
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| Subject |
Engineering Materials
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| Description |
In the present study, pristine magnesium aluminium layered double hydroxide (Mg-Al LDH, sample A) nanoparticles were synthesized by simple co-precipitation technique followed by intercalation of methotrexate drug (MTX), their detailed physicochemical characterization and development of RP-HPLC method for estimation of MTX intercalated in Mg-Al LDH nanoparticles (sample B). The PXRD analysis showed the basal spacing of 003 plane corresponding to sample A and sample B to be 8.63 angstrom and 11.39 angstrom, respectively. Particle size distributions were measured to be 56-75 nm (D-50=66.15 nm) in case of sample A and 75-100 nm (D-50=87.41 nm) for sample B. Simple, accurate RP-HPLC method was developed for estimation of the MTX drug from sample B, on Agilent HPLC 1200 system with UV detector on L1 column. 0.02 M potassium dihydrogen phosphate solution (pH 3.0) and acetonitrile (85:15 v/v) was used as mobile phase in an isocratic elution mode at a flow rate of 1.0 mL/min at 35 degrees C with a load of 10 mu L at 307 nm. The retention time for methotrexate was at 4.44 min. The method was validated as per ICH Q2 (R1) guidelines. The method was found to be linear over a concentration range of 10-100 mu g/mL.
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| Publisher |
Indian Ceramic Society
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| Date |
2021-04
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://cgcri.csircentral.net/5325/1/jui.pdf
Basu, Arindam and Ray, Sayantan and Saha, Suman and Chakraborty, Jui (2021) Development and Validation of RP-HPLC Method for Estimation of Methotrexate Drug Intercalated in Mg-Al Layered Double Hydroxide Nanoparticles. Transactions of the Indian Ceramic Society, 80 (2). pp. 127-134. ISSN 0371-750X |
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| Relation |
http://cgcri.csircentral.net/5325/
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