Regulation Of Cell Signaling By Inter-Organeller Interaction In Neuronal And Non-Neuronal Cells: Effect On MicroRNA Mediated Gene Regulation
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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Title |
Regulation Of Cell Signaling By Inter-Organeller
Interaction In Neuronal And Non-Neuronal Cells:
Effect On MicroRNA Mediated Gene Regulation
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Creator |
DE, DIPAYAN
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Subject |
Molecular & Human Genetics
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Description |
Uponexposuretoamyloid-β oligomers(Aβ1–42), glialcellsstart
expressingproinflammatorycytokines,despiteanincreaseinlevels
of repressivemicroRNAs(miRNAs).Exploringthemechanismof
thispotentialimmunityoftargetcytokinemRNAsagainstrepressive
miRNAsinamyloid-β-exposedglialcells,wehaveidentifieddifferential
compartmentalizationofrepressivemiRNAsinglialcellsthatexplains
thisaberrantmiRNAfunction.InAβ1–42-treatedcells,whereastarget
mRNAswerefoundtobeassociatedwithpolysomesattachedto
endoplasmicreticulum(ER),themiRNAribonucleoproteincomplexes
(miRNPs)werefoundtobepresentpredominantlywithendosomes
thatfailedtorecycletoER-attachedpolysomes,preventingrepression
of mRNAtargets.Aβ1–42 oligomers,bymaskingRab7aproteinson
endosomalsurfaces,affectedRab7ainteractionwithRab-interacting
lysosomalprotein(RILP),restrictingthelysosomaltargetingand
recyclingofmiRNPs.RNA-processingbody(P-body)localizationof
themiRNPswasfoundtobeenhancedinamyloid-β-treatedcellsas
a consequenceofenhancedendosomalretentionofmiRNPs.
Interestingly,depletionofP-bodycomponentspartlyrescuedthe
miRNAfunctioninglialcellsexposedtoamyloid-β andrestrictedthe
excesscytokineexpression.
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Date |
2021
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Type |
Thesis
NonPeerReviewed |
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Format |
application/pdf
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Identifier |
http://www.eprints.iicb.res.in/2829/1/Thesis_for_Evaluation_DIPAYAN_DE.pdf
DE, DIPAYAN (2021) Regulation Of Cell Signaling By Inter-Organeller Interaction In Neuronal And Non-Neuronal Cells: Effect On MicroRNA Mediated Gene Regulation. PhD thesis, UNIVERSITY OF CALCUTTA. |
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Relation |
http://www.eprints.iicb.res.in/2829/
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