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Regulation Of Cell Signaling By Inter-Organeller Interaction In Neuronal And Non-Neuronal Cells: Effect On MicroRNA Mediated Gene Regulation

IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata

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Field Value
 
Title Regulation Of Cell Signaling By Inter-Organeller Interaction In Neuronal And Non-Neuronal Cells: Effect On MicroRNA Mediated Gene Regulation
 
Creator DE, DIPAYAN
 
Subject Molecular & Human Genetics
 
Description Uponexposuretoamyloid-β oligomers(Aβ1–42), glialcellsstart expressingproinflammatorycytokines,despiteanincreaseinlevels of repressivemicroRNAs(miRNAs).Exploringthemechanismof thispotentialimmunityoftargetcytokinemRNAsagainstrepressive miRNAsinamyloid-β-exposedglialcells,wehaveidentifieddifferential compartmentalizationofrepressivemiRNAsinglialcellsthatexplains thisaberrantmiRNAfunction.InAβ1–42-treatedcells,whereastarget mRNAswerefoundtobeassociatedwithpolysomesattachedto endoplasmicreticulum(ER),themiRNAribonucleoproteincomplexes (miRNPs)werefoundtobepresentpredominantlywithendosomes thatfailedtorecycletoER-attachedpolysomes,preventingrepression of mRNAtargets.Aβ1–42 oligomers,bymaskingRab7aproteinson endosomalsurfaces,affectedRab7ainteractionwithRab-interacting lysosomalprotein(RILP),restrictingthelysosomaltargetingand recyclingofmiRNPs.RNA-processingbody(P-body)localizationof themiRNPswasfoundtobeenhancedinamyloid-β-treatedcellsas a consequenceofenhancedendosomalretentionofmiRNPs. Interestingly,depletionofP-bodycomponentspartlyrescuedthe miRNAfunctioninglialcellsexposedtoamyloid-β andrestrictedthe excesscytokineexpression.
 
Date 2021
 
Type Thesis
NonPeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/2829/1/Thesis_for_Evaluation_DIPAYAN_DE.pdf
DE, DIPAYAN (2021) Regulation Of Cell Signaling By Inter-Organeller Interaction In Neuronal And Non-Neuronal Cells: Effect On MicroRNA Mediated Gene Regulation. PhD thesis, UNIVERSITY OF CALCUTTA.
 
Relation http://www.eprints.iicb.res.in/2829/