Somatic embryogenesis in Asparagus densiflorus (Kunth) Jessop cv Sprengeri
Metadata of CSIR Papers
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| Title |
Somatic embryogenesis in Asparagus densiflorus (Kunth) Jessop cv Sprengeri
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| Creator |
Dasgupta, CN
Mukhopadhyay, MJ Mukhopadhyay, S |
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| Subject |
Biochemistry & Molecular Biology; Plant Sciences
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| Description |
An efficient protocol has been developed for plant regeneration in Asparagus densiflorus (Kunth) Jessop cv Sprengeri (Asparagaceae) through somatic embryogenesis from spear sections. Callus culture was initiated on Murashige and Skoog (MS) medium formulation containing alpha-naphthaleneacetic acid (5.37 mu M), indole-3-acetic acid (5.71 mu M) and 6benzylaminopurine (2.22 mu M). The callus became embryogenic by transferring to 2, 4-dichlorophenoxyacetic acid (4.52 mu M) containing MS medium. Somatic embryos developed and matured vigorously on MS medium with NAA (1.07 mu M), 6-gamma-gamma-dimethylaminopurine (9.84 mu M) and abscisic acid (1.89 mu M). Mature bipolar embryos were converted efficiently into plants on MS medium in the presence of low level of kinetin (2.32 mu M). Regenerated plants showed 80% survival after transfer to field. These plants were all diploid (2n=60). Peroxidase activity was maximum in the embryogenic callus as documented from the gel as well as spectro photometric analysis.
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| Publisher |
SOC PLANT BIOCHEM BIOTECHNEW DELHIDIV BIOCHEM, INDIAN AGRICULTURAL RES INST, NEW DELHI 110 012, INDIA
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| Date |
2011-09-20T12:12:32Z
2011-09-20T12:12:32Z 2007 |
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| Type |
Article
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| Identifier |
JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY
0971-7811 http://hdl.handle.net/123456789/14311 |
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| Language |
English
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