Protein tyrosine phosphorylation of a heparin-binding sperm membrane mitogen (HBSM) is associated with capacitation and acrosome reaction
Metadata of CSIR Papers
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Title |
Protein tyrosine phosphorylation of a heparin-binding sperm membrane mitogen (HBSM) is associated with capacitation and acrosome reaction
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Creator |
Mor, V
Das, T Bhattacharjee, M Chatterjee, T |
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Subject |
Biochemistry & Molecular Biology; Biophysics
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Description |
Protein tyrosine phosphorylation in spermatozoa is associated with epididymal maturation and though to be central for attainment of a capacitated state and expression of hyperactivated motility. Heparin, the most highly sulfated glycosaminoglycans, was also the most potent at stimulating the acrosomal reaction in bovine epididymal spermatozoa. Studies using radiolabeled inorganic phosphate showed 11-fold increase (32)Pi incorporation in heparin-binding sperm membrane protein (HBSM) during spermatozoal capacitation, and the phosphorylation occurs at the tyrosine residue. Epididymal spermatozoa were induced to undergo capacitation and acrosome reaction by 70% when the cells were incubated in BWW medium supplemented with heparin. The spermatozoa pre-treated with anti-HBSM antibody showed 46% reduction in the hyperactivated motility and lowers the acrosome reaction. This was confirms by measuring the hydrolysis of benzoyl-L-arginine ethyl ether (BAEE) by the acrosomal enzyme; acrosin. The preliminary finding suggests that HBSM may play an important role in the sperm capacitation and acrosome reaction. (c) 2006 Elsevier Inc. All rights reserved.
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Publisher |
ACADEMIC PRESS INC ELSEVIER SCIENCESAN DIEGO525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA
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Date |
2011-09-20T12:12:42Z
2011-09-20T12:12:42Z 2007 |
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Type |
Article
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Identifier |
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
0006-291X http://hdl.handle.net/123456789/14384 |
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Language |
English
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