In silico comparative study of the genomic islands of Vibrio cholerae MJ1236 with those of Classical and El TorN16961 strains of Vibrio cholerae
IR@IICB: CSIR-Indian Institute of Chemical Biology, KolkataView Archive Info
In silico comparative study of the genomic islands of Vibrio cholerae
MJ1236 with those of Classical and El TorN16961 strains of
Kundu, Jayanta Kumar
Molecular & Human Genetics
The evolution of microbial genomes is greatly influenced by horizontal gene
transfer (HGT), where large blocks of horizontally acquired foreign sequences,
often encoding virulence determinants, occur in chromosomes of pathogenic
bacteria. A program DESIGN-ISLAND developed in our laboratory was used on three
completely sequenced Vibrio cholerae genomes, V. cholerae Classical O395, El Tor
N16961 and MJ1236, in order to identify the putative horizontally acquired
regions. The putative genomic islands (GIs) were graphically represented and
analyzed. The study identified distinct regions in the GIs of V. cholerae MJ1236
which were shared either with the Classical or the El Tor strain of V. cholerae. A
cluster comprising of 38 ORFs was common to V. cholerae strains of MJ1236 and
Classical O395 but absent in El Tor N16961. About 5% of the predicted GIs of V.
cholerae MJ1236 were unique to itself. Among these unique ORFs, a region of
mostly hypothetical genes was identified, where the ORFs were present in a large
cluster. The results show that the HGT had played a significant role in the
evolution and the differentiation of V. cholerae MJ1236.
Dutta, Avirup and Kundu, Jayanta Kumar and Chatterjee, Raghunath and Chaudhuri, Keya (2011) In silico comparative study of the genomic islands of Vibrio cholerae MJ1236 with those of Classical and El TorN16961 strains of Vibrio cholerae. FEMS Microbiology Letters, 321. pp. 75-81.