Recent Progress on Phospholipases: Different Sources, Assay Methods, Industrial Potential and Pathogenicity
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Recent Progress on Phospholipases: Different Sources,
Assay Methods, Industrial Potential and Pathogenicity
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| Creator |
Ramrakhiani, Lata
Chand, Subhash |
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| Subject |
Drug Development/Diagnostics & Biotechnology
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| Description |
Significant studies on phospholipases optimization, characterization, physiological
role and industrial potential have been conducted worldwide. Some of them have been
directed for biotechnological advances such as gene discovery and functional enhancement
by protein engineering. Others reported phospholipases as virulence factor and major cause
of pathophysiological effects. A general overview on phospholipase is needed for the
identification of new reliable and efficient phospholipase, which would be potentially used
in number of industrial and medical applications. Phospholipases catalyse the hydrolysis of
one or more ester and phosphodiester bonds of glycerophospholipids. They vary in site of
action on phospholipid which can be used industrially for modification/production of new
phospholipids. Catalytically active phospholipase mainly use phosphatidylcholine as major
substrate, but they can also show specificity with other phospholipids. Several accurate
phospholipase assay methods are known, but a rapid and reliable method for highthroughput
screening is still a challenge for efficient supply of superior phospholipases and
their practical applications. Major application of phospholipase is in industries like oil
refinery, health food manufacturing, dairy, cosmetics etc. All types of phospholipases can
be involved as virulence factor. They can also be used as diagnostic markers for microbial
infection. The importance of phospholipase in virulence is proven and inhibitors of the
enzyme can be used as candidate for preventing the associated disease.
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| Date |
2011
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/35/1/APPLIED_BIOCHEMISTRY_AND_BIOTECHNOLOGY%2C_Volume_164%2C_Issue_7%2C_Pages_991%2D102%2C2011[15].pdf
Ramrakhiani, Lata and Chand, Subhash (2011) Recent Progress on Phospholipases: Different Sources, Assay Methods, Industrial Potential and Pathogenicity. Appl Biochem Biotechnol, 164 (7). pp. 991-1022. |
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| Relation |
http://dx.doi.org/10.1007/s12010-011-9190-6
http://www.eprints.iicb.res.in/35/ |
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