Homologous Liver Parenchymal Cell-Cell Adhesion Mediated by an Endogenous Lectin and its Receptor
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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Title |
Homologous Liver Parenchymal Cell-Cell Adhesion
Mediated by an Endogenous Lectin and its Receptor
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Creator |
Banerjee, Saswti
Majumder, Gopal Chandra |
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Subject |
Cell Biology & Physiology
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Description |
Many studies have implicated cell-surface lectins in heterologous
cell-cell adhesion, but little is known about the participation of lectins in cellular
adhesion in homologous cells. Here, we show the development of a cell model
for investigating the direct role of a cell-surface lectin in homologous cell-cell
adhesion. Parenchymal cells were isolated from caprine liver using a perfusion
buffer, and dispersed in a chemically defined modified Ringer’s solution. These
cells undergo autoagglutination in the presence of Ca2+. The autoagglutinated
cells can be dissociated specifically with D-galactose (50 mM), which also
inhibits the liver cell autoagglutination event. The blood serum protein fetuin has
no effect on liver cell autoagglutination, whereas desialylated fetuin (100 μM),
with its terminal D-galactose residue, showed a high affinity for blocking the
autoagglutination event. The data demonstrates the occurrence of a Ca2+-dependent
D-galactose-specific lectin and a lectin receptor on the parenchymal cells.
Furthermore, it shows that the observed autoagglutination event is caused by the
interaction of the cell-surface lectin with its receptor on the neighbouring
homologous cells. The data supports the view that homologous cell-cell contact
in mammalian tissues is triggered by such lectin-receptor interaction and that the
previously reported cell-surface adhesive proteins serve as a secondary force to
strengthen cell adhesion. This cell model could be extremely useful for
investigating the direct role of cell-surface lectin and its receptor in homologous
cell adhesion in a variety of tissues under normal and pathological conditions.
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Date |
2010
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Type |
Article
PeerReviewed |
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Format |
application/pdf
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Identifier |
http://www.eprints.iicb.res.in/65/1/CELLULAR_%26_MOLECULAR_BIOLOGY_LETTERS_15(2)356%2D364_;2010[79].pdf
Banerjee, Saswti and Majumder, Gopal Chandra (2010) Homologous Liver Parenchymal Cell-Cell Adhesion Mediated by an Endogenous Lectin and its Receptor. Cellular & Molecular Biology Letters, 15. pp. 356-364. |
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Relation |
http://dx.doi.org/10.2478/s11658-010-0011-7
http://www.eprints.iicb.res.in/65/ |
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