Curcumin Alleviates Matrix Metalloproteinase-3 and -9 Activities during Eradication of Helicobacter pylori Infection in Cultured Cells and Mice
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Curcumin Alleviates Matrix Metalloproteinase-3 and -9
Activities during Eradication of Helicobacter pylori
Infection in Cultured Cells and Mice
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| Creator |
Kundu, Parag
De, Ronita Pal, Ipsita Mukhopadhyay, Asish K Saha, Dhira Rani Swarnakar, Snehasikta |
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| Subject |
Drug Development/Diagnostics & Biotechnology
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| Description |
Current therapy-regimens against Helicobacter pylori (Hp) infections have considerable failure rates and adverse side effects
that urge the quest for an effective alternative therapy. We have shown that curcumin is capable of eradicating Hp-infection
in mice. Here we examine the mechanism by which curcumin protects Hp infection in cultured cells and mice. Since, MMP-3
and -9 are inflammatory molecules associated to the pathogenesis of Hp-infection, we investigated the role of curcumin on
inflammatory MMPs as well as proinflammatory molecules. Curcumin dose dependently suppressed MMP-3 and -9
expression in Hp infected human gastric epithelial (AGS) cells. Consistently, Hp-eradication by curcumin-therapy involved
significant downregulation of MMP-3 and -9 activities and expression in both cytotoxic associated gene (cag)+ve and cag-ve
Hp-infected mouse gastric tissues. Moreover, we demonstrate that the conventional triple therapy (TT) alleviated MMP-3
and -9 activities less efficiently than curcumin and curcumin’s action on MMPs was linked to decreased pro-inflammatory
molecules and activator protein-1 activation in Hp-infected gastric tissues. Although both curcumin and TT were associated
with MMP-3 and -9 downregulation during Hp-eradication, but unlike TT, curcumin enhanced peroxisome proliferatoractivated
receptor-c and inhibitor of kappa B-a. These data indicate that curcumin-mediated healing of Hp-infection
involves regulation of MMP-3 and -9 activities.
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| Date |
2011
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/68/1/PLOS_ONE%2C6(1)%2C2011[94].pdf
Kundu, Parag and De, Ronita and Pal, Ipsita and Mukhopadhyay, Asish K and Saha, Dhira Rani and Swarnakar, Snehasikta (2011) Curcumin Alleviates Matrix Metalloproteinase-3 and -9 Activities during Eradication of Helicobacter pylori Infection in Cultured Cells and Mice. PLoS ONE, 6 (1). |
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| Relation |
http://dx.doi.org/10.1371/journal.pone.0016306.g004
http://www.eprints.iicb.res.in/68/ |
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