Sialic Acids in Different Leishmania sp., its Correlation with Nitric Oxide Resistance and Host Responses
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Sialic Acids in Different Leishmania sp., its Correlation with Nitric Oxide Resistance and Host Responses
|
|
| Creator |
Ghoshal, Angana
Gerwig, Gerrit J Kamerling, Johannis P Mandal, Chitra |
|
| Subject |
Infectious Diseases and Immunology
|
|
| Description |
The presence of different derivatives of sialic acids (SA)
on Leishmania donovani instigated us to investigate their
status on different strains of Leishmania sp. causing different
forms of the disease. Leishmania tropica (K27),
Leishmania major (JISH118) and Leishmania mexicana
(LV4) responsible for cutaneous, Leishmania braziliensis
(L280) and Leishmania amazonensis (LV81) causing diffuse
and Leishmania infantum (MON29) responsible for
visceral leishmaniasis were included in this study. The
strains showed a differential distribution of SA in spite
of their close resemblance in pathogenesis. K27,
JISH118, L280 and MON29 were categorized as high
SA-containing strains having enhanced 9-O-acetyl sialic
acid (9-O-AcSAhigh) whereas LV4 and LV81 evidenced
considerably reduced SA. Interestingly, 9-O-AcSAhigh
promastigotes showed significant viability as compared
to their de-O-acetylated forms after exposure to NaNO2
suggesting the involvement of 9-O-AcSA in conferring
nitric oxide (NO) resistance. Enhanced intracellular survivability
was demonstrated following infection of human
macrophages with 9-O-AcSAhigh promastigotes in contrast
to their de-O-acetylated forms indicating their
contribution in bestowing a survival benefit. Additionally,
reduced accumulation of NO, interleukin-12 and
interferon-γ in the supernatant of macrophages infected
with 9-O-AcSAhigh promastigotes indicated suppression
of leishmanicidal host responses. However, LV4 and
LV81 with least 9-O-AcSA, before and after de-O-acetylation,
showed unaltered NO resistance, multiplicity and
host responses signifying the probable involvement of
other determinants which may be a function of their
inherent parasitic attribute. Hence, enhanced levels of
9-O-AcSA serve as one of the potential determinants responsible
for increased NO resistance and survivability of
parasites by inhibition of host responses.
|
|
| Date |
2010
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://www.eprints.iicb.res.in/79/1/GLYCOBIOLOGY_20(5)_553%2D566_;2010[85].pdf
Ghoshal, Angana and Gerwig, Gerrit J and Kamerling, Johannis P and Mandal, Chitra (2010) Sialic Acids in Different Leishmania sp., its Correlation with Nitric Oxide Resistance and Host Responses. Glycobiology, 20 (5). pp. 553-566. |
|
| Relation |
http:/dx.doi.org/10.1093/glycob/cwp207
http://www.eprints.iicb.res.in/79/ |
|