A fadD mutant of Vibrio cholerae Is Impaired in the Production of Virulence Factors and Membrane Localization of the Virulence Regulatory Protein TcpP�
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
A fadD mutant of Vibrio cholerae Is Impaired in the Production of
Virulence Factors and Membrane Localization of the
Virulence Regulatory Protein TcpP�
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| Creator |
Ray, Sreejana
Chatterjee, Epshita Chatterjee, Arpita Paul, Kalidas Chowdhury, Rukhsana |
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| Subject |
Infectious Diseases and Immunology
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| Description |
hierarchical expression of several regulatory proteins comprising the ToxR regulon. In this study, we demonstrate
that disruption of the fadD gene encoding a long-chain fatty acyl coenzyme A ligase has marked effects
on expression of the ToxR virulence regulon, motility, and in vivo lethality of V. cholerae. In the V. cholerae fadD
mutant, expression of the major virulence genes ctxAB and tcpA, encoding cholera toxin (CT), and the major
subunit of the toxin-coregulated pilus (TCP) was drastically repressed and a growth-phase-dependent reduction
in the expression of toxT, encoding the transcriptional activator of ctxAB and tcpA, was observed.
Expression of toxT from an inducible promoter completely restored CT to wild-type levels in the V. cholerae fadD
mutant, suggesting that FadD probably acts upstream of toxT expression. Expression of toxT is activated by the
synergistic effect of two transcriptional regulators, TcpP and ToxR. Reverse transcription-PCR and Western
blot analysis indicated that although gene expression and production of both TcpP and ToxR are unaffected
in the fadD mutant strain, membrane localization of TcpP, but not ToxR, is severely impaired in the fadD
mutant strain from the mid-logarithmic phase of growth. Since the decrease in toxT expression occurred
concomitantly with the reduction in membrane localization of TcpP, a direct correlation between the defect in
membrane localization of TcpP and reduced toxT expression in the fadD mutant strain is suggested.
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| Date |
2011
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/106/1/INFECTION_AND_IMMUNITY%2C79(1)%2C_258%2D266%2C2011[113].pdf
Ray, Sreejana and Chatterjee, Epshita and Chatterjee, Arpita and Paul, Kalidas and Chowdhury, Rukhsana (2011) A fadD mutant of Vibrio cholerae Is Impaired in the Production of Virulence Factors and Membrane Localization of the Virulence Regulatory Protein TcpP�. Infection and Immunity, 79 (1). pp. 258-266. |
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| Relation |
http://dx.doi.org/10.1128/IAI.00663-10
http://www.eprints.iicb.res.in/106/ |
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