Antiparkinsonian Effects of Aqueous Methanolic Extract of Hyoscyamus niger Seeds Result From its Monoamine Oxidase Inhibitory and Hydroxyl Radical Scavenging Potency
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Antiparkinsonian Effects of Aqueous Methanolic Extract
of Hyoscyamus niger Seeds Result From its Monoamine Oxidase
Inhibitory and Hydroxyl Radical Scavenging Potency
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| Creator |
Sengupta, T
Vinayagam, J Nagashayana, N Gowda, B Jaisankar, P Mohanakumar, K P |
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| Subject |
Cell Biology & Physiology
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| Description |
Hyoscyamus species is one of the four plants
used in Ayurveda for the treatment of Parkinson’s disease
(PD). Since Hyoscyamus niger was found to contain negligible
levels of L-DOPA, we evaluated neuroprotective
potential, if any, of characterized petroleum ether and
aqueous methanol extracts of its seeds in 1-methyl-4-phenyl-
1,2,3,6-tetrahydropyridine (MPTP) model of PD in
mice. Air dried authenticated H. niger seeds were
sequentially extracted using petroleum ether and aqueous
methanol and were characterized employing HPLC-electrochemistry
and LCMS. Parkinsonian mice were treated
daily twice with the extracts (125–500 mg/kg, p.o.) for two
days and motor functions and striatal dopamine levels were
assayed. Administration of the aqueous methanol extract
(containing 0.03% w/w of L-DOPA), but not petroleum ether extract, significantly attenuated motor disabilities
(akinesia, catalepsy and reduced swim score) and striatal
dopamine loss in MPTP treated mice. Since the extract
caused significant inhibition of monoamine oxidase activity
and attenuated 1-methyl-4-phenyl pyridinium (MPP?)-
induced hydroxyl radical (�OH) generation in isolated
mitochondria, it is possible that the methanolic extract of
Hyoscyamus niger seeds protects against parkinsonism in
mice by means of its ability to inhibit increased �OH
generated in the mitochondria.
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| Date |
2011
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/110/1/NEUROCHEMICAL_RESEARCH%2C_36(1)_177%2D186%2C2011[110].pdf
Sengupta, T and Vinayagam, J and Nagashayana, N and Gowda, B and Jaisankar, P and Mohanakumar, K P (2011) Antiparkinsonian Effects of Aqueous Methanolic Extract of Hyoscyamus niger Seeds Result From its Monoamine Oxidase Inhibitory and Hydroxyl Radical Scavenging Potency. Neurochem Res, 36 (1). pp. 177-186. |
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| Relation |
http://dx.doi.org/10.1007/s11064-010-0289-x
http://www.eprints.iicb.res.in/110/ |
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