Spindle Assembly Checkpoint Protein Cdc20 Transcriptionally Activates Expression of Ubiquitin Carrier Protein UbcH10*□
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Spindle Assembly Checkpoint Protein Cdc20 Transcriptionally
Activates Expression of Ubiquitin Carrier Protein UbcH10*□
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| Creator |
Nath, Somsubhra
Banerjee, Taraswi Sen, Debrup Das, Tania Roychoudhury, Susanta |
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| Subject |
Molecular & Human Genetics
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| Description |
The spindle assembly checkpoint (SAC) ensures accurate segregation
of chromosomes by monitoring kinetochore attachment
of spindles during mitosis. Proper progression of mitosis
depends on orderly ubiquitination and subsequent degradation
of various mitotic inhibitors. At the molecular level, upon
removal of SAC, Cdc20 activates E3 ubiquitin ligase anaphasepromoting
complex/cyclosome that, along with E2 ubiquitinconjugating
enzyme UbcH10, executes this function. Both
Cdc20 and UbcH10 are overexpressed in many cancer types and
are associated with defective SAC function leading to chromosomal
instability. The precise mechanism of correlated overexpression
of these two proteins remains elusive. We show that
Cdc20 transcriptionally up-regulates UbcH10 expression. The
WD40 domain of Cdc20 is required for this activity. Physical
interaction between Cdc20 and anaphase-promoting complex/
cyclosome-CBP/p300 complex and its subsequent recruitment
to the UBCH10 promoter are involved in this transactivation
process. This transcriptional regulatory function of Cdc20 was
observed to be cell cycle-specific. We hypothesize that this coregulated
overexpression of both proteins contributes to chromosomal
instability.
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| Publisher |
American Society for Biochemistry and Molecular Biology
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| Date |
2011
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/111/1/JOURNAL_OF_BIOLOGICAL_CHEMISTRY%2C_286(18)%2C2011_[51].pdf
Nath, Somsubhra and Banerjee, Taraswi and Sen, Debrup and Das, Tania and Roychoudhury, Susanta (2011) Spindle Assembly Checkpoint Protein Cdc20 Transcriptionally Activates Expression of Ubiquitin Carrier Protein UbcH10*□. The Journal of Biological Chemistry, 286 (18). pp. 15666-15677. |
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| Relation |
http://dx.doi.org/:10.1186/1476-4598-9-58
http://www.eprints.iicb.res.in/111/ |
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