Interaction of the Anticancer Plant Alkaloid Sanguinarine with Bovine Serum Albumin
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Interaction of the Anticancer Plant Alkaloid Sanguinarine
with Bovine Serum Albumin
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| Creator |
Hossain, Maidul
Khan, Asma Yasmeen Kumar, G Suresh |
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| Subject |
Chemistry
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| Description |
Background: Interaction of the iminium and alkanolamine forms of sanguinarine with bovine serum albumin (BSA) was
characterized by spectroscopic and calorimetric techniques.
Methodology/Principal Findings: Formation of strong complexes of BSA with both iminium and alkanolamine forms was
revealed from fluorescence quenching of sanguinarine. Binding parameters calculated from Stern-Volmer quenching
method revealed that the neutral alkanolamine had higher affinity to BSA compared to the charged iminium form. Specific
binding distances of 3.37 and 2.38 nm between Trp 212 (donor) and iminium and alkanolamine forms (acceptor),
respectively, were obtained from Forster resonance energy transfer studies. Competitive binding using the site markers
warfarin and ibuprofen, having definite binding sites, demonstrated that both forms of sanguinarine bind to site I
(subdomain IIA) on BSA. Sanguinarine binding alters protein conformation by reducing the a-helical organization and
increasing the coiled structure, indicating a small but definitive partial unfolding of the protein. Thermodynamic parameters
evaluated from isothermal titration calorimetry suggested that the binding was enthalpy driven for the iminium form but
favoured by negative enthalpy and strong favourable entropy contributions for the alkanolamine form, revealing the
involvement of different molecular forces in the complexation.
Conclusions/Significance: The results suggest that the neutral alkanolamine form binds to the protein more favourably
compared to the charged iminium, in stark contrast to the reported DNA binding preference of sanguinarine.
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| Date |
2011
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/123/1/PLOS_ONE%2C_6(4)%2C2011[65].pdf
Hossain, Maidul and Khan, Asma Yasmeen and Kumar, G Suresh (2011) Interaction of the Anticancer Plant Alkaloid Sanguinarine with Bovine Serum Albumin. PLOS ONE, 6 (4). |
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| Relation |
http://dx.doi.org/10.1371/journal.pone.0018333
http://www.eprints.iicb.res.in/123/ |
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