Nicotiana Tabacum Overexpressing c-ECS Exhibits Biotic Stress Tolerance likely through NPR1-Dependent Salicylic Acid-Mediated Pathway
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Nicotiana Tabacum Overexpressing c-ECS Exhibits Biotic Stress
Tolerance likely through NPR1-Dependent Salicylic Acid-Mediated Pathway
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| Creator |
Ghanta, Srijani
Bhattacharyya, Dipto Sinha, Ragini Banerjee, Anindita Chattopadhyay, Sharmila |
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| Subject |
Drug Development/Diagnostics & Biotechnology
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| Description |
The elaborate networks and the crosstalk of
established signaling molecules like salicylic acid (SA),
jasmonic acid (JA), ethylene (ET), abscisic acid (ABA),
reactive oxygen species (ROS) and glutathione (GSH) play
key role in plant defense response. To obtain further insight
into the mechanism through which GSH is involved in this
crosstalk to mitigate biotic stress, transgenic Nicotiana
tabacum overexpressing Lycopersicon esculentum gammaglutamylcysteine
synthetase (LeECS) gene (NtGB lines)
were generated with enhanced level of GSH in comparison
with wild-type plants exhibiting resistance to pathogenesis
as well. The expression levels of non-expressor of pathogenesis-
related genes 1 (NPR1)-dependent genes like
pathogenesis-related gene 1 (NtPR1), mitogen-activated
protein kinase kinase (NtMAPKK), glutamine synthetase
(NtGLS) were significantly enhanced alongwith NtNPR1.
However, the expression levels of NPR1-independent
genes like NtPR2, NtPR5 and short-chain dehydrogenase/
reductase family protein (NtSDRLP) were either insignificant
or were downregulated. Additionally, increase in
expression of thioredoxin (NtTRXh), S-nitrosoglutathione
reductase 1 (NtGSNOR1) and suppression of isochorismate
synthase 1 (NtICS1) was noted. Comprehensive analysis of
GSH-fed tobacco BY2 cell line in a time-dependent manner reciprocated the in planta results. Better tolerance of
NtGB lines against biotrophic Pseudomonas syringae pv.
tabaci was noted as compared to necrotrophic Alternaria
alternata. Through two-dimensional gel electrophoresis
(2-DE) and image analysis, 48 differentially expressed
spots were identified and through identification as well as
functional categorization, ten proteins were found to be
SA-related. Collectively, our results suggest GSH to be a
member in cross-communication with other signaling
molecules in mitigating biotic stress likely through NPR1-
dependent SA-mediated pathway.
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| Date |
2011
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/124/1/PLANTA%2C_233(5)_%2C_895%2D910%2C2011_[63].pdf
Ghanta, Srijani and Bhattacharyya, Dipto and Sinha, Ragini and Banerjee, Anindita and Chattopadhyay, Sharmila (2011) Nicotiana Tabacum Overexpressing c-ECS Exhibits Biotic Stress Tolerance likely through NPR1-Dependent Salicylic Acid-Mediated Pathway. Planta, 235 (5). pp. 895-910. |
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| Relation |
http://dx.doi.org/10.1007/s00425-011-1349-4
http://www.eprints.iicb.res.in/124/ |
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