Short COMMUNICATION Cytochrome P450 Inhibition Assay for Standardized Extract of Terminalia chebula Retz.ptr_2993 151..154
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Short COMMUNICATION
Cytochrome P450 Inhibition Assay for
Standardized Extract of Terminalia
chebula Retz.ptr_2993 151..154
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| Creator |
Ponnusankar, S
Pandit, S Venkatesh, M Bandyopadhyay, Arun Mukherjee, Pulok K |
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| Subject |
Cell Biology & Physiology
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| Description |
The hydroalcoholic extract of fruit pulp of Terminalia chebula Retz. was standardized and evaluated for its
safety through cytochrome P450 (CYP 450) inhibition assay. Standardization was performed through high
performance thin layer chromatography (HPTLC) using gallic acid (GA) standard. Cytochrome P450-CO
complex microplate assay was performed using rat liver microsomes. The effect of standardized extract, its
fraction and bioactive marker compound were comparatively evaluated for its effect on CYP P450 enzymes.The
extract of fruit pulp was used for HPTLC,where the Rf value of the marker was found to be 0.43.The calibration
plot was linear in the range of 2–14 mg of GA and correlation co-efficient of 0.99965. The mean quantity of GA
was found to be 2.5% w/w.The CYP P450 concentration of the rat liver microsome sample used in the study was
found to be 0.417 nmol/mg protein.The in vitro effect of various concentrations of extracts and fractions showed
a linear concentration-dependent inhibition of cytochrome P450 up to 60 mL. The study showed more inhibition
of fraction when compared to the extract and GA. Still, the inhibition showed by fraction is less when compared
with standard Ketoconazole. Thus, this study indicated the in vitro cytochrome P450 inhibition potential of T.
Chebula. Copyright © 2010 John Wiley & Sons, Ltd.
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| Date |
2011
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/125/1/PHYTOTHERAPY_RESEARCH%2C25_(1)%2C151%2D154%2C2011[111].pdf
Ponnusankar, S and Pandit, S and Venkatesh, M and Bandyopadhyay, Arun and Mukherjee, Pulok K (2011) Short COMMUNICATION Cytochrome P450 Inhibition Assay for Standardized Extract of Terminalia chebula Retz.ptr_2993 151..154. PHYTOTHERAPY RESEARCH, 25 (1). pp. 151-154. |
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| Relation |
http://dx.doi.org/10.1002/ptr.2993
http://www.eprints.iicb.res.in/125/ |
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