Genetic Transformation of Bacopa Monnieri by Wild type Strains of Agrobacterium Rhizogenes Stimulates Production of Bacopa Saponins in Transformed Calli and Plants
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Genetic Transformation of Bacopa Monnieri by Wild type Strains
of Agrobacterium Rhizogenes Stimulates Production of Bacopa
Saponins in Transformed Calli and Plants
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| Creator |
Majumder, Sukanya
Garai, Saraswati Jha, Sumita |
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| Subject |
Chemistry
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| Description |
We have developed an efficient transformation
system for Bacopa monnieri, an important Indian medicinal
plant, using Agrobacterium rhizogenes strains LBA 9402
and A4. Transformed roots induced by strain LBA 9402
spontaneously dedifferentiated to callus while excised roots
induced by strain A4 spontaneously showed induction of
shoot buds within 10 days. PCR and RT-PCR analysis
revealed the presence and expression of the rolAB and rolC
genes at the transcription level in pRi A4 transformed cultures
indicating that the TL-DNA was integrated retained
and expressed in the A4-Ri transformed shoots. Transformed
calli showed the presence of rolAB or rol A, TR and
ags genes. Transformed plants showed morphological features
typically seen in transgenic plants produced by
A. rhizogenes. Growth and biomass accumulation was significantly
higher in the transformed shoots (twofold) and
roots (fourfold) than in the non-transformed (WT) plants. In
pRi A4-transformed plants, the content of bacopasaponin D,
bacopasaponin F, bacopaside II and bacopaside V was
enhanced significantly as compared to WT plants of similar
age while bacoside A3 and bacopasaponin C content was
comparable with that of WT plants. Significant increase in content of five bacopa saponins could be detected in pRi
9402-transformed callus cultures. There is an overall
stimulatory effect on accumulation of bacopa saponins in
transformed plants and cells of B. monnieri establishing
the role of endogenous elicitation by Ri T-DNA of
A. rhizogenes.
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| Publisher |
Springer Verlag
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| Date |
2011
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/126/1/PLANT_CELL_REPORTS%2C30(_5)_941%2D954%2C2011[62].pdf
Majumder, Sukanya and Garai, Saraswati and Jha, Sumita (2011) Genetic Transformation of Bacopa Monnieri by Wild type Strains of Agrobacterium Rhizogenes Stimulates Production of Bacopa Saponins in Transformed Calli and Plants. Plant Cell Reports, 30 (5). pp. 941-954. |
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| Relation |
http://dx.doi.org/10.1007/s00299-011-1035-9
http://www.eprints.iicb.res.in/126/ |
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