Protection of peroxiredoxin II on oxidative stress-induced cardiomyocyte death and apoptosis
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Protection of peroxiredoxin II on oxidative
stress-induced cardiomyocyte death and
apoptosis
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| Creator |
Zhao, Wen
Fan, Guo-Chang Zhang, Zhi-Guo Bandyopadhyay, Arun Zhou, Xiaoyang Kranias, Evangelia G |
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| Subject |
Cell Biology & Physiology
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| Description |
Peroxiredoxin II, a cytosolic isoform of the antioxidant
enzyme family, has been implicated in cancer-associated cell death and
apoptosis, but its functional role in the heart remains to be elucidated.
Interestingly, the expression levels of peroxiredoxin II were decreased in
mouse hearts upon ischemia-reperfusion, while they were elevated in two
genetically modified hyperdynamic hearts with phospholamban ablation
or protein phosphatase 1 inhibitor 1 overexpression. To delineate the
functional significance of altered peroxiredoxin II expression, adenoviruses
encoding sense or antisense peroxiredoxin II were generated;
cardiomyocytes were infected, and then subjected to H2O2 treatment to
mimic oxidative stress-induced cell death and apoptosis. H2O2 stimulation
resulted in a significant decrease of endogenous peroxiredoxin II
expression, along with reduced cell viability in control cells. However,
overexpression of peroxiredoxin II significantly protected from H2O2-
induced apoptosis and necrosis, while downregulation of this enzyme
promoted the detrimental effects of oxidative stress in cardiomyocytes.
The beneficial effects of peroxiredoxin II were associated with increased
Bcl-2 expression, decreased expression of Bax and attenuated activity of
caspases 3, 9 and 12. Furthermore, there were no significant alterations in
the expression levels of the other five isoforms of peroxiredoxin, as well
as active catalase or glutathione peroxidase-1 after ischemia-reperfusion
or H2O2 treatment. These findings suggest that peroxiredoxin II may be a
unique antioxidant in the cardiac system and may represent a potential
target for cardiac protection from oxidative stress-induced injury
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| Publisher |
Springer Verlag
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| Date |
2009
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/133/1/BASIC_RESEARCH_IN_CARDIOLOGY%2C104(4_)__377%2D389%2C2009[55].pdf
Zhao, Wen and Fan, Guo-Chang and Zhang, Zhi-Guo and Bandyopadhyay, Arun and Zhou, Xiaoyang and Kranias, Evangelia G (2009) Protection of peroxiredoxin II on oxidative stress-induced cardiomyocyte death and apoptosis. Basic Research in Cardiology, 104. pp. 377-389. |
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| Relation |
http://www.eprints.iicb.res.in/133/
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