Alterations in Candidate Genes PHF2, FANCC, PTCH1 and XPA at Chromosomal 9q22.3 Region: Pathological Significance in Early- and Late-onset Breast Carcinoma
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Alterations in Candidate Genes PHF2, FANCC, PTCH1 and XPA at
Chromosomal 9q22.3 Region: Pathological Significance in Early- and Late-onset Breast Carcinoma
|
|
| Creator |
Sinha, Satyabrata
Singh, Ratnesh Kumar Alam, Neyaz Roy, Anup Roychoudhury, Susanta Panda, Chinmay Kumar |
|
| Subject |
Molecular & Human Genetics
|
|
| Description |
Introduction: Younger women with breast carcinoma (BC) exhibits more aggressive pathologic features
compared to older women; young age could be an independent predictor of adverse prognosis. To find any
existing differences in the molecular pathogenesis of BC in both younger and older women, alterations at
chromosomal (chr.) 9q22.32-22.33 region were studied owing to its association in wide variety of tumors. Present
work focuses on comparative analysis of alterations of four candidate genes; PHF2, FANCC, PTCH1 and XPA
located within 4.4 Mb region of the afore-said locus in two age groups of BC, as well as the interrelation and
prognostic significance of alterations of these genes.
Methods: Deletion analysis of PHF2, FANCC, PTCH1 and XPA were examined in a subset of 47 early-onset
(group-A: ≤ 40 years) and 59 late-onset (group-B: > 40 years) breast carcinomas using both microsatellite and
exonic markers. Methylation Sensitive Restriction analysis (MSRA) was done to check for promoter methylation.
Quantitative real-time polymerase chain reaction (Q-PCR) and immunohistochemisty (IHC) was done in some
genes to see their relative mRNA and protein expressions respectively. Clinico-pathological correlation of
different parameters as well as patient survival was calculated using different statistical softwares like EpiInfo 6.04b, SPSS 10.0 etc. Results: Either age group exhibited high frequency of overall alterations in PHF2, FANCC and PTCH1 compared to XPA. Samples with alteration (deletion/methylation) in these genes showed reduced level of mRNA expression as seen by Q-PCR. Immunohistochemical analysis of FANCC and PTCH1 also supported this observation. Poor patient survival was noted in both age groups having alterations in FANCC. Similar result was also seen with PTCH1 and XPA alterations in group-A and PHF2 alterations in group-B. This reflected their roles as prognostic tools in the respective groups in which they were altered. Conclusion: Overall alterations of PHF2, FANCC and PTCH1 were comparatively higher than XPA. Differential association of alterations in FANCC and PTCH1 with that of PHF2, XPA and two breast cancer susceptibility
genes (BRCA1/BRCA2) in the two age groups suggests differences in their molecular pathogenesis and
dysregulation of multiple DNA repair pathways as well as hedgehog dependent stem cell renewal pathway.
|
|
| Date |
2008
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://www.eprints.iicb.res.in/154/1/MOLECULAR_CANCER%2C7_(84)%2C2008[17].pdf
Sinha, Satyabrata and Singh, Ratnesh Kumar and Alam, Neyaz and Roy, Anup and Roychoudhury, Susanta and Panda, Chinmay Kumar (2008) Alterations in Candidate Genes PHF2, FANCC, PTCH1 and XPA at Chromosomal 9q22.3 Region: Pathological Significance in Early- and Late-onset Breast Carcinoma. Molecular Cancer, 7 (84). |
|
| Relation |
http://dx.doi.org/10.1186/1476-4598-7-84
http://www.eprints.iicb.res.in/154/ |
|