Study of Laccase Production by Pleurotus Ostreatus in a 5 l Bioreactor and Application of the Enzyme to Determine the Antioxidant Concentration of Human Plasma
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Study of Laccase Production by Pleurotus Ostreatus in a 5 l
Bioreactor and Application of the Enzyme to Determine the
Antioxidant Concentration of Human Plasma
|
|
| Creator |
Mazumder, S
Bose, S Bandopadhyay, A Alam, S Mukherjee, M |
|
| Subject |
Cell Biology & Physiology
|
|
| Description |
Aims: To achieve high laccase production from Pleurotus ostreatus in a bench top bioreactor and to utilize the enzyme for determination of the total antioxidant
concentration (TAC) of human plasma. Methods and Results: Laccase production by P. ostreatus studied in a benchtop
bioreactor was as high as, 874Æ0 U ml)1 in presence of copper sulfate. The enzyme was used to replace metmyoglobin and hydrogen peroxide for the estimation of TAC in human plasma. The trolox equivalent antioxidant concentrations
determined by the laccase-based method and metmyoglobin method ranged from 1Æ63 ± 0Æ011 to 1Æ80 ± 0Æ006 mmol l)1 and from 1Æ41 ± 0Æ004 to 1Æ51 ± 0Æ008 mmol l)1 plasma, respectively. Conclusions: Pleurotus ostreatus produced high amount of extracellular laccase in a benchtop bioreactor. The enzyme can be used to assay TAC of blood
plasma without the interference encountered with the hydrogen peroxide and metmyoglobin mediated assay method.
Significance and Impact of the Study: Laccase production by P. ostreatus obtained in this study was the highest among all reported laccase producing white-rot fungi. Moreover, an accurate laccase-based assay method was developed
for detection of TAC in human plasma.
|
|
| Date |
2008
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://www.eprints.iicb.res.in/163/1/LETTERS_IN_APPLIED_MICROBIOLOGY%2C47(_4)%2C_355%2D360%2C2008[26].pdf
Mazumder, S and Bose, S and Bandopadhyay, A and Alam, S and Mukherjee, M (2008) Study of Laccase Production by Pleurotus Ostreatus in a 5 l Bioreactor and Application of the Enzyme to Determine the Antioxidant Concentration of Human Plasma. Letters in Applied Microbiology, 47 (4). pp. 355-360. |
|
| Relation |
http://dx.doi.org/10.1111/j.1472-765X.2008.02418.x
http://www.eprints.iicb.res.in/163/ |
|