Intestinal Adherence of Vibrio cholerae Involves a Coordinated Interaction between Colonization Factor GbpA and Mucin�
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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Title |
Intestinal Adherence of Vibrio cholerae Involves a Coordinated Interaction between Colonization Factor GbpA and Mucin�
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Creator |
Bhowmick, Rudra
Ghosal, Abhisek Das, Bhabatosh Koley, Hemanta Saha, Dhira Rani Ganguly, Sandipan Nandy, Ranjan K Bhadra, Rupak K Chatterjee, Nabendu Sekhar |
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Subject |
Infectious Diseases and Immunology
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Description |
The chitin-binding protein GbpA of Vibrio cholerae has been recently described as a common adherence
factor for chitin and intestinal surface. Using an isogenic in-frame gbpA deletion mutant, we first show that V.
cholerae O1 El Tor interacts with mouse intestinal mucus quickly, using GbpA in a specific manner. The gbpA
mutant strain showed a significant decrease in intestinal adherence, leading to less colonization and fluid
accumulation in a mouse in vivo model. Purified recombinant GbpA (rGbpA) specifically bound to N-acetyl-
D-glucosamine residues of intestinal mucin in a dose-dependent, saturable manner with a dissociation constant
of 11.2 �M. Histopathology results from infected mouse intestine indicated that GbpA binding resulted in a
time-dependent increase in mucus secretion. We found that rGbpA increased the production of intestinal
secretory mucins (MUC2, MUC3, and MUC5AC) in HT-29 cells through upregulation of corresponding genes.
The upregulation of MUC2 and MUC5AC genes was dependent on NF-�B nuclear translocation. Interestingly,
mucin could also increase GbpA expression in V. cholerae in a dose-dependent manner. Thus, we propose that
there is a coordinated interaction between GbpA and mucin to upregulate each other in a cooperative manner,
leading to increased levels of expression of both of these interactive factors and ultimately allowing successful
intestinal colonization and pathogenesis by V. cholerae.
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Date |
2008
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Type |
Article
PeerReviewed |
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Format |
application/pdf
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Identifier |
http://www.eprints.iicb.res.in/175/1/INFECTION_AND_IMMUNITY%2C76(_11)%2C4968%2D4977%2C2008[19].pdf
Bhowmick, Rudra and Ghosal, Abhisek and Das, Bhabatosh and Koley, Hemanta and Saha, Dhira Rani and Ganguly, Sandipan and Nandy, Ranjan K and Bhadra, Rupak K and Chatterjee, Nabendu Sekhar (2008) Intestinal Adherence of Vibrio cholerae Involves a Coordinated Interaction between Colonization Factor GbpA and Mucin�. Infection and Immunity, 76 (11). pp. 4968-4977. |
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Relation |
http://dx.doi.org/10.1128/IAI.01615-07
http://www.eprints.iicb.res.in/175/ |
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