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Genetic transformation of a hepatoprotective plant, Phyllanthus amarus

IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata

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Title Genetic transformation of a hepatoprotective plant, Phyllanthus amarus
Creator Banerjee, Anindita
Chattopadhyay, Sharmila
Subject Drug Development/Diagnostics & Biotechnology
Description Phyllanthus amarus Schum & Thonn. is a source of various pharmacologically active compounds such as phyllanthin, hypophyllanthin, gallic acid, catechin, and nirurin, a flavone glycoside. A genetic transformation method using Agrobacterium tumefaciens was developed for this plant species for the first time. Shoot tips of full grown plants were used as explants for Agrobacteriummediated transformation. Transgenic plants were obtained by co-cultivation of shoot tips explants and A. tumefaciens strain LBA4404 containing the pCAMBIA 2301 plasmid harboring neomycin phosphotransferase II (NPT II) and β- glucuronidase encoding (GUS) genes in the T-DNA region in the presence of 200 μM acetosyringone. Integration of the NPT II gene into the genome of transgenic plants was verified by PCR and Southern blot analyses. Expression of the NPT II gene was confirmed by RT-PCR analysis. An average of 25 explants was used, out of which an average of 19 explants produced kanamycin-resistant shoots, which rooted to produce 13 complete transgenic plants.
Publisher Society for In-Vitro Biology
Date 2009
Type Article
Format application/pdf
Identifier http://www.eprints.iicb.res.in/185/1/IN_VITRO_CELLULAR_%26_DEVELOPMENTAL_BIOLOGY%2DPLANT%2C45(1)%2C__57%2D64%2C_2009[108].pdf
Banerjee, Anindita and Chattopadhyay, Sharmila (2009) Genetic transformation of a hepatoprotective plant, Phyllanthus amarus. In Vitro Cellular and Developmental Biology - Plant, 45 (1). pp. 57-64. ISSN 1054-5476
Relation http://dx.doi.org/10.1007/s11627-008-9160-z