Genetic transformation of a hepatoprotective plant, Phyllanthus amarus
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Genetic transformation of a hepatoprotective
plant, Phyllanthus amarus
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| Creator |
Banerjee, Anindita
Chattopadhyay, Sharmila |
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| Subject |
Drug Development/Diagnostics & Biotechnology
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| Description |
Phyllanthus amarus Schum & Thonn. is a source
of various pharmacologically active compounds such as
phyllanthin, hypophyllanthin, gallic acid, catechin, and
nirurin, a flavone glycoside. A genetic transformation
method using Agrobacterium tumefaciens was developed
for this plant species for the first time. Shoot tips of full
grown plants were used as explants for Agrobacteriummediated
transformation. Transgenic plants were obtained
by co-cultivation of shoot tips explants and A. tumefaciens
strain LBA4404 containing the pCAMBIA 2301 plasmid
harboring neomycin phosphotransferase II (NPT II) and β-
glucuronidase encoding (GUS) genes in the T-DNA region
in the presence of 200 μM acetosyringone. Integration of
the NPT II gene into the genome of transgenic plants was
verified by PCR and Southern blot analyses. Expression of
the NPT II gene was confirmed by RT-PCR analysis. An
average of 25 explants was used, out of which an average
of 19 explants produced kanamycin-resistant shoots, which
rooted to produce 13 complete transgenic plants.
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| Publisher |
Society for In-Vitro Biology
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| Date |
2009
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/185/1/IN_VITRO_CELLULAR_%26_DEVELOPMENTAL_BIOLOGY%2DPLANT%2C45(1)%2C__57%2D64%2C_2009[108].pdf
Banerjee, Anindita and Chattopadhyay, Sharmila (2009) Genetic transformation of a hepatoprotective plant, Phyllanthus amarus. In Vitro Cellular and Developmental Biology - Plant, 45 (1). pp. 57-64. ISSN 1054-5476 |
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| Relation |
http://dx.doi.org/10.1007/s11627-008-9160-z
http://www.eprints.iicb.res.in/185/ |
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