Small Chromosomal Integration Site of Classical CTX Prophage in Mozambique Vibrio Cholerae O1 Biotype El Tor Strain
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Small Chromosomal Integration Site of Classical CTX Prophage
in Mozambique Vibrio Cholerae O1 Biotype El Tor Strain
|
|
| Creator |
Das, Bhabatosh
Halder, Kalpataru Pal, Partha Bhadra, Rupak K |
|
| Subject |
Infectious Diseases and Immunology
|
|
| Description |
An unusual strain of Vibrio cholerae O1 biotype
El Tor harbouring multiple tandem copies of classical
CTX prophage caused a cholera epidemic in Mozambique
in 2004. However, the location of the classical CTX prophage
in the genome of the Mozambique strain was
unknown. In this study, pulsed Weld gel electrophoresis
(PFGE) of the whole genome along with Southern hybridization
experiments indicated that the classical CTX prophage
present in the Mozambique strain is located in the
small chromosome. To determine the CTX prophage integration
site in the small chromosome of Mozambique
strain, the 5_and 3_ junctions of the prophage and small
chromosome were PCR ampliWed, cloned and sequenced.
Sequence analysis indicated that the prophage was integrated
in the conserved dif site of the replication terminus
region of the Mozambique strain. While using an O1 El Tor
isolate VC44 as a control strain, which carries tandem copies
of CTX prophage in its small chromosome like the
Mozambique strain, it was unexpectedly detected that the
strain VC44 also possesses classical cholera toxin B gene
allele. Since the strain VC44 was isolated in India in the
year 1992, it appears that the Mozambique strain has probably
originated from a VC44-like strain.
|
|
| Date |
2007
|
|
| Type |
Article
PeerReviewed |
|
| Format |
application/pdf
|
|
| Identifier |
http://www.eprints.iicb.res.in/206/1/ARCHIVES_OF_MICROBIOLOGY%2C188(_6)%2C_677%2D683%2C2007_[14].pdf
Das, Bhabatosh and Halder, Kalpataru and Pal, Partha and Bhadra, Rupak K (2007) Small Chromosomal Integration Site of Classical CTX Prophage in Mozambique Vibrio Cholerae O1 Biotype El Tor Strain. Arch Microbiol, 188. pp. 677-683. |
|
| Relation |
http://dx.doi.org/10.1007/s00203-007-0275-0
http://www.eprints.iicb.res.in/206/ |
|