Evaluation of cell types for assessment of cytogenetic damage in arsenic exposed population
IR@IICB: CSIR-Indian Institute of Chemical Biology, KolkataView Archive Info
Evaluation of cell types for assessment of cytogenetic damage in
arsenic exposed population
Singh, Keshav K
Giri, Ashok K
Molecular & Human Genetics
Background: Cytogenetic biomarkers are essential for assessing environmental exposure, and
reflect adverse human health effects such as cellular damage. Arsenic is a potential clastogen and
aneugen. In general, the majority of the studies on clastogenic effects of arsenic are based on
frequency of micronuclei (MN) study in peripheral lymphocytes, urothelial and oral epithelial cells.
To find out the most suitable cell type, here, we compared cytogenetic damage through MN assay
in (a) various populations exposed to arsenic through drinking water retrieved from literature
review, as also (b) arsenic-induced Bowen's patients from our own survey.
Results: For literature review, we have searched the Pubmed database for English language journal
articles using the following keywords: "arsenic", "micronuclei", "drinking water", and "human" in
various combinations. We have selected 13 studies consistent with our inclusion criteria that
measured micronuclei in either one or more of the above-mentioned three cell types, in human
samples. Compared to urothelial and buccal mucosa cells, the median effect sizes measured by the
difference between people with exposed and unexposed, lymphocyte based MN counts were found
to be stronger. This general pattern pooled from 10 studies was consistent with our own set of
three earlier studies. MN counts were also found to be stronger for lymphocytes even in arsenicinduced
Bowen's patients (cases) compared to control individuals having arsenic-induced noncancerous
Conclusion: Overall, it can be concluded that MN in lymphocytes may be superior to other
epithelial cells for studying arsenic-induced cytogenetic damage.
Ghosh, Pritha and Basu, Arindam and Singh, Keshav K and Giri, Ashok K (2008) Evaluation of cell types for assessment of cytogenetic damage in arsenic exposed population. Molecular Cancer, 7 (45).