Structural and Functional Characterization and Physiological Significance of a Stimulator Protein of Mg2+-independent Ca2+-ATPase Isolated from Goat Spermatozoa
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Structural and Functional Characterization and Physiological
Significance of a Stimulator Protein of Mg2+-independent
Ca2+-ATPase Isolated from Goat Spermatozoa
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| Creator |
Sengupta, Tanusree
Ghoshal, Srabasti Dungdung, Sandhya Rekha Majumder, Gopal Chandra Sen, Parimal C |
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| Subject |
Cell Biology & Physiology
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| Description |
Recently a low-molecular-mass protein purified
from goat testes cytosol has been reported from our
laboratory which is found to stimulate Mg2+-independent
Ca2+-ATPase without any significant effect on Mg2+-
dependent Ca2+-ATPase. In the present study, detailed
structural and functional characterization, as well as the
physiological significance of the protein has been described.
The stimulatory effect is found to be inhibited by known
inhibitors of P-type ATPases, vanadate and lanthanum
chloride. Monitoring of the phosphoenzyme intermediate by
autoradiography has shown that the stimulation of the
ATPase is due to the enhancement in the rate of dephosphorylation
of the overall reaction step. Along with the
stimulation of the enzyme activity, the protein is found to
enhance the calcium uptake. Amino acid analysis data show
that the stimulator contains about 26% non-polar amino acid
facilitating easy penetration to the hydrophobic core of the
membrane bound ATPase. Circular dichroism analysis of the
protein suggested the presence of all secondary structural
elements. The Western-blotting experiment shows its
expression level is the highest in goat testes. Peptide fragments
obtained in MALDI-MS analysis when subjected to
MSDB database search by MASCOT search engine reveals
that the proteins of close similarity with the protein under
study are actin related protein 2/3 complex subunit, peptidylprolyl
cis-trans isomerase and gastrin releasing peptide precursor. Besides, the protein under study is also shown to
decrease the forward motility of goat sperm without having
any significant effect on the total motility indicating its
possible role in fertility regulation.
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| Date |
2008
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/210/1/MOLECULAR_AND_CELLULAR_BIOCHEMISTRY%2C311(1%2D2)%2C93%2D103%2C2008[105].pdf
Sengupta, Tanusree and Ghoshal, Srabasti and Dungdung, Sandhya Rekha and Majumder, Gopal Chandra and Sen, Parimal C (2008) Structural and Functional Characterization and Physiological Significance of a Stimulator Protein of Mg2+-independent Ca2+-ATPase Isolated from Goat Spermatozoa. Mol Cell Biochem, 311 (1-2). pp. 93-103. |
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| Relation |
http://dx.doi.org/10.1007/s11010-007-9700-6
http://www.eprints.iicb.res.in/210/ |
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