Enhanced Production of Antimicrobial Compounds by Three Salt-Tolerant Actinobacterial Strains Isolated from the Sundarbans in a Niche-Mimic Bioreactor
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Enhanced Production of Antimicrobial Compounds
by Three Salt-Tolerant Actinobacterial Strains Isolated
from the Sundarbans in a Niche-Mimic Bioreactor
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| Creator |
Sarkar, Sreyashi
Saha, Malay Roy, Debashis Jaisankar , P Das, Satada Roy, Lalita Gauri Gachhui, Ratan Sen, Tuhinadri Mukherjee, Joydeep |
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| Subject |
Chemistry
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| Description |
A novel reactor system, the rotating disk bioreactor
(RDBR), was used to mimic the niche environmental
conditions of three salt-tolerant estuarine actinobacteria
isolated from the Sundarbans region off the Bay of Bengal,
designated MS310 (99% similar in its 16S rRNA gene
sequence to Streptomyces parvallus), MS3/20 and MS1/7.
The RDBR, operated at a rotational speed of one revolution
per day, 50% submergence of discs, aeration rate of 1.0 vvm,
and with a sucrose-containing medium, faithfully mimicked
the intertidal estuarine habitat of these marine isolates, and
supported biofilm formation and production of antimicrobial
metabolites—in particular, actinomycin D by MS310. Onset
of antibiotic production by MS310 occurs at 20 h in the
RDBR compared to 55 h in a conventional stirred-tank
bioreactor (STBR). Furthermore, peak antimicrobial activity is attained much earlier in the RDBR with MS310 (at 45 h)
than that reported with a terrestrial strain of S. parvallus
grown in a STBR (at 144 h). Peak antimicrobial activity of
metabolites produced by MS1/7 and MS3/20 were also
attained earlier in the RDBR (at 25 and 12 h, respectively)
than in a STBR (at 80 and 28 h, respectively). Antibiotic
synthesis in the three isolates, in general, appears to be
associated with their growth. Overall, the RDBR may be
considered the preferred alternative to the STBR for
production of antimicrobials by biofilm-forming estuarine
bacteria for its much higher surface/volume ratio, lower
costs, and easy operability.
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| Date |
2008
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/212/1/MARINE_BIOTECHNOLOGY%2C10(_5)%2C518%2D526%2C2008[42].pdf
Sarkar, Sreyashi and Saha, Malay and Roy, Debashis and Jaisankar , P and Das, Satada and Roy, Lalita Gauri and Gachhui, Ratan and Sen, Tuhinadri and Mukherjee, Joydeep (2008) Enhanced Production of Antimicrobial Compounds by Three Salt-Tolerant Actinobacterial Strains Isolated from the Sundarbans in a Niche-Mimic Bioreactor. Mar Biotechnol, 10 (5). pp. 518-526. |
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| Relation |
http://dx.doi.org/10.1007/s10126-008-9090-0
http://www.eprints.iicb.res.in/212/ |
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