Stimulation of Salmon Calcitonin on Secretion of 17b-Estradiol by the Ovarian Follicles of Common Carp, Cyprinus Carpio
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Stimulation of Salmon Calcitonin on Secretion of 17b-Estradiol by the Ovarian Follicles of Common Carp, Cyprinus Carpio
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| Creator |
Paul, Sudipta
Mukherjee, Dola Pramanick, Kousik Kundu, Sourav Bhattacharyya, S P De, Priyanka Mukherjee, Dilip |
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| Subject |
Molecular & Human Genetics
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| Description |
The effects of salmon calcitonin (sCT) on the secretion of
17b-estradiol (E2) were examined in female common carp,
Cyprinus carpio. Vitellogenic stage fish adapted to high-Ca
water were i.p. injected with vehicle, sCT, human chorionic
gonadotropin (hCG), or hCG plus sCT. To determine
whether ovarian follicles are equipped with CT receptors, a
CT binding assay was conducted. In the in vitro experiments,
vitellogenic follicles were incubated with stimulators and
inhibitors. Administration of sCT increased the basal and
hCG-stimulated E2 release in vivo and in vitro. Binding
characteristics of [125I]sCT to plasma membrane preparation
of carp ovarian follicles showed saturability with high-affinity
(KdZ48.48 pmol/l and BmaxZ1.2 pmol/mg protein). To
clarify the mechanism of E2 production by sCT, in vitro effect
of sCT and hCG on aromatase activity (conversion of
testosterone to E2) and cytochrome P450 aromatase (P450arom) gene expression in carp ovarian follicles were
investigated. Salmon CT-stimulated both aromatase activity
and P450arom gene expression in ovarian follicles of carp.
sCT-stimulated E2 release by the ovarian follicles in vitro was
augmented in the presence of dibutyryl cAMP. Inhibitor of
protein kinase A (PKA), SQ 22536 inhibited sCT-stimulated
steroid production in a dose-dependent manner. Specific
inhibitor of protein kinase C (PKC), NPC-15437 dihydrochloride
had no inhibitory effects on sCT-induced E2
release. The present study indicates that sCT binds specifically
to carp ovary and stimulates E2 production by increasing the
activity of cytochrome P450 aromatase and P450arom gene
expression. The results further suggest that stimulatory action
of sCTon E2 production is mediated through cAMP pathway.
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| Date |
2008
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/233/1/JOURNAL_OF_ENDOCRINOLOGY%2C196(2)%2C413%2D424%2C2008[130].pdf
Paul, Sudipta and Mukherjee, Dola and Pramanick, Kousik and Kundu, Sourav and Bhattacharyya, S P and De, Priyanka and Mukherjee, Dilip (2008) Stimulation of Salmon Calcitonin on Secretion of 17b-Estradiol by the Ovarian Follicles of Common Carp, Cyprinus Carpio. Journal of Endocrinology, 196 (2). pp. 413-424. |
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| Relation |
http://dx.doi.org/10.1677/JOE-07-0188
http://www.eprints.iicb.res.in/233/ |
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