Functional Analysis of the Essential GTP-Binding-Protein-Coding Gene cgtA of Vibrio cholerae�
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Functional Analysis of the Essential GTP-Binding-Protein-Coding Gene cgtA of Vibrio cholerae�
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| Creator |
Shah, Sangita
Das, Bhabatosh Bhadra, Rupak K |
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| Subject |
Infectious Diseases and Immunology
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| Description |
Vibrio cholerae, the causative agent of the severe diarrheal
disease cholera, is a fast-growing organism. Among various
regulatory proteins involved in bacterial growth management,
the essential GTP-binding proteins have been shown to play
critical roles, although the exact mechanisms are yet to be
elucidated. The GTP-binding protein CgtA, coded by the cgtA
gene, belonging to Obg subfamily has been found to be highly
conserved and essential in prokaryotes (14, 15, 17, 20, 22, 23, 27). Studies conducted with different prokaryotic organisms have suggested functional roles of the CgtA/Obg protein in various physiological processes, like regulation of initiation of sporulation (30), DNA replication (15, 26), chromosome partitioning (14), replication fork stability (7), chromosome segregation (8), ribosome maturation (12, 25), and maintaining the steady-state level of ppGpp during exponential growth (13). It has been demonstrated that Escherichia coli temperature-
sensitive obgE (cgtA) mutant cells form long filaments
with expanded, nonpartitioned chromosomes (14). Foti et al.
(7) isolated an obgE mutant that was sensitive to various replication inhibitors, like hydroxyurea (HU). Further studies by the same group showed that ObgE is required for cell cycle progression in E. coli (8). Sato et al. (25) recently showed that ObgE is associated with several ribosomal proteins. Thus, in E. coli CgtA/ObgE appears to be involved in ribosome biogenesis. In Vibrio harveyi, an important species of the genus Vibrio, CgtA is an essential protein associated with the 50S ribosomal subunit.
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| Date |
2008
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/237/1/JOURNAL_OF_BACTERIOLOGY%2C190(13)%2C4764%2D4771%2C2008[61].pdf
Shah, Sangita and Das, Bhabatosh and Bhadra, Rupak K (2008) Functional Analysis of the Essential GTP-Binding-Protein-Coding Gene cgtA of Vibrio cholerae�. African Journal of Biotechnology, 190 (13). pp. 4764-4771. |
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| Relation |
http://dx.doi.org/10.1128/JB.02021-07
http://www.eprints.iicb.res.in/237/ |
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