Molecular characterization of Echinococcus granulosus of Indian animal isolates on the basis of nuclear and mitochondrial genotype
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Molecular characterization of Echinococcus granulosus of Indian
animal isolates on the basis of nuclear and mitochondrial genotype
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| Creator |
Gudewar, J
Pan, D Bera, A. K Das, S. K Konar, Aditya Rao, J.R |
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| Subject |
Chemistry
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| Description |
Sixty-six isolates of larval stage of Echinococcus
granulosus, a known pathogenic parasite of man
and animals were collected from cattle, buffalo, sheep, and
goats. Single-stranded conformation polymorphism (SSCP)
for analysis of variation after denaturation of amplicon of
intron of actin II (ACTII) revealed six SSCP phenotypes.
Intron portion was analyzed considering introns-early and
introns-late theories. Isolates belonging to different conformers
were further screened for mitochondrial ATPase
subunit 6 (ATP6) and NADH dehydrogenase subunit II
(nadII) genotypes. Assignment of each isolate to its specific
strain was achieved after comparing with standard
genotypes of E. granulosus. Variants deduced by nuclear
targets did not match with mitochondrial haplotypes. A
possible explanation for this observation can be attributed
toward interspecific hybridization since cross-fertilization
occurs less frequently in hermaphrodite organisms. A
phylogenetic tree drawn on the basis of predicted aminoacid
sequence of ATP6 and nadII revealed two distinct clusters i.e. E. granulosus sensu stricto and E. ortleppi/
cattle strain (EG5). To the best of our knowledge, this is the
first report of genetic characterization of two distinct ATP6
and nadII genotypes of zoonotic importance living in
sympatry.
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| Publisher |
Kluwer
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| Date |
2009
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/243/1/MOLECULAR_BIOSYSTEMS%2C5(3)%2C_244%2D254%2C2009[125].pdf
Gudewar, J and Pan, D and Bera, A. K and Das, S. K and Konar, Aditya and Rao, J.R (2009) Molecular characterization of Echinococcus granulosus of Indian animal isolates on the basis of nuclear and mitochondrial genotype. Molecular Biology Reports, 36 (6). pp. 1381-1385. |
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| Relation |
http://www.eprints.iicb.res.in/243/
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