Transformed Hairy Roots of Arachis hypogea: A Tool for Studying Root Nodule Symbiosis in a Non–Infection Thread Legume of the Aeschynomeneae Tribe
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Transformed Hairy Roots of Arachis hypogea: A Tool
for Studying Root Nodule Symbiosis
in a Non–Infection Thread Legume
of the Aeschynomeneae Tribe
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| Creator |
Sinharoy, Senjuti
Saha, Sudip Roychoudhury, Susanta DasGupta, Maitrayee |
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| Subject |
Molecular & Human Genetics
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| Description |
Arachis hypogea is a non–“infection thread” (IT) legume
where rhizobial entry or dissemination in the nodules never
involves IT. Rhizobia invade through epidermal “cracks”
and directly access the cortical cells to develop the characteristic
aeschynomenoid nodules. For investigating these
nonclassical nodulation features in Arachis spp., we developed
an efficient procedure for Agrobacterium rhizogenes
R1000-mediated transformation of this plant. In this study,
we optimized the induction of hairy roots and nodulation
of composite Arachis hypogea plants in the presence of Bradyrhizobium
sp. (Arachis) strain NC92. 35S promoterdriven
green fluorescent protein and β-glucuronidase expression
indicated transformation frequency to be above
80%. The transformed roots had the characteristic rosettetype
root hairs and had normal level of expression of symbiosis-
related genes SymRK and CCaMK. The transgenic
nodules resembled the wild-type nodules with an exception
of 2 to 3%, where they structurally deviated from the wildtype
nodules to form nodular roots. A 16S rRNA profile of
an infected-zone metagenome indicated that identical populations
of bradyrhizobia invaded both composite wild-type
plants grown in natural soil. Our results demonstrate that
Arachis hairy root is an attractive system for undertaking
investigations of the nonclassical features associated with
its nitrogen-fixing symbiotic interactions
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| Date |
2009
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/247/1/MOLECULAR_PLANT%2DMICROBE_INTERACTIONS%2C_22(2)%2C132%2D142%2C_2009[110].pdf
Sinharoy, Senjuti and Saha, Sudip and Roychoudhury, Susanta and DasGupta, Maitrayee (2009) Transformed Hairy Roots of Arachis hypogea: A Tool for Studying Root Nodule Symbiosis in a Non–Infection Thread Legume of the Aeschynomeneae Tribe. Molecular Plant-Microbe Interacitions, 22 (2). pp. 132-142. |
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| Relation |
http://www.eprints.iicb.res.in/247/
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