9-O-acetylated sialic acids enhance entry of virulent Leishmania donovani promastigotes into macrophages
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
9-O-acetylated sialic acids enhance entry of virulent
Leishmania donovani promastigotes into macrophages
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| Creator |
Ghoshal, Angana
Mukhopadhyay, S Chava, A. K Gerwig, G. J Kamerling, Johannis P Chatterjee, M Mandal, Chitra |
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| Subject |
Infectious Diseases and Immunology
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| Description |
Distribution of 9-O-acetylated sialic acids (9-O-AcSA) on Leishmania donovani has been previously reported. Considering
their role in recognition, the differential distribution of sialic acids especially 9-O-acetylated sialic acids in avirulent (UR6)
versus virulent (AG83 and GE1) promastigotes of Leishmania donovani and its role in entry into macrophages was explored.
Fluorimetric-HPLC, fluorimetric determination and ELISA revealed 14-, 8- and 5-fold lower sialic acids in UR6 as
compared to AG83. Interestingly, on UR6, flow cytometry indicated lower (a2p6)-linked sialoglycoproteins along with
minimal 9-O-acetylated sialoglycoproteins by Scatchard analysis. Further, UR6 demonstrated a 9- and 14.5-fold lower
infectivity and phagocytic index than AG83. Additionally, de-O-acetylation and de-sialylation of AG83 demonstrated a
3- and 1.5-fold reduced phagocytic index. The role of 9-O-AcSA in entry was further confirmed by pre-blocking the
macrophage surface with a cocktail of sugars followed by microscopic quantification. The phagocytic index of AG83
exclusively through 9-O-AcSA was significantly high. Interestingly, AG83 produced higher metacyclic promastigotes
containing increased 9-O-AcSA as compared to avirulent UR6 supporting its virulent nature. Taken together; our results
conclusively demonstrate the increased presence of 9-O-acetylated sialic acid on promastigotes of virulent Leishmania
donovani as compared to avirulent UR6 and their subsequent role in entry within macrophages.
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| Publisher |
Cambridge University Press
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| Date |
2009
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/253/1/PARASITOLOGY%2C136(2)%2C159%2D173%2C2009[113].pdf
Ghoshal, Angana and Mukhopadhyay, S and Chava, A. K and Gerwig, G. J and Kamerling, Johannis P and Chatterjee, M and Mandal, Chitra (2009) 9-O-acetylated sialic acids enhance entry of virulent Leishmania donovani promastigotes into macrophages. Parasitology, 136 (2). pp. 159-173. |
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| Relation |
http://dx.doi.org/10.1017/S0031182008005180
http://www.eprints.iicb.res.in/253/ |
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