Assessment of the effect of Bacopa monnieri (L.) Wettst. extract on the labeling of blood elements with technetium-99m and on the morphology of red blood cells
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Assessment of the effect of Bacopa monnieri (L.) Wettst. extract
on the labeling of blood elements with technetium-99m and on the
morphology of red blood cells
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| Creator |
De, Kakali
Chandra, Susmita Misra, Mridula |
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| Subject |
Infectious Diseases and Immunology
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| Description |
Bacopa monnieri (L.) Wettst. (BM), a traditional Ayurvedic medicine, used for
centuries as a memory enhancing, anti-inflammatory, antipyretic, sedative and antiepileptic agent.
BM extract have been extensively investigated by several authors for their neuropharmacological
effects. In nuclear medicine, red blood cells (RBC) labeled with technetium-99m (99mTc) have
several clinical applications. However, data have demonstrated that synthetic or natural drugs could
modify the labeling of RBC with 99mTc. As Bacopa monnieri is extensively used in medicine, we
evaluated its influence on the labeling of RBC and plasma proteins using technetium-99m (99mTc).
This labeling procedure depends on a reducing agent and usually stannous chloride is used. Blood
was incubated with BM extracts. Stannous chloride solution and 99mTc were added. Blood was
centrifuged and plasma (P) and blood cells (BC) were isolated. Samples of P or BC were also
precipitated, centrifuged and insoluble fraction (IF) and soluble fraction (SF) were separated. The
percentage of radioactivity (%ATI) in BC, IF-BC and IF-P were calculated. The %ATI significantly
decreased on BC from 95.53±0.45 to 35.41±0.44, on IF-P from 80.20±1.16 to 7.40±0.69 and
on IF-BC from 73.31±1.76 to 21.26±1.40. The morphology study of RBC revealed important
morphological alterations due to treatment with BM extracts. We suggest that the BM extract effect
could be explained by an inhibition of the stannous and pertechnetate ions or oxidation of the
stannous ion or by damages induced in the plasma membrane
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| Date |
2009
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/256/1/REVISTA_BRASILEIRA_DE_FARMACOGNOSIA%2DBRAZILIAN_JOURNAL_OF_PHARMACOGNOSY%2C19(_3)%2C664%2D671%2C2009[54].pdf
De, Kakali and Chandra, Susmita and Misra, Mridula (2009) Assessment of the effect of Bacopa monnieri (L.) Wettst. extract on the labeling of blood elements with technetium-99m and on the morphology of red blood cells. Revista Brasileira de Farmacognosia Brazilian Journal of Pharmacognosy, 19 (3). pp. 664-671. |
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| Relation |
http://dx.doi.org/10.1590/S0102-695X2009000500003
http://www.eprints.iicb.res.in/256/ |
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