Transcriptional Upregulation of Inflammatory Cytokines in Human Intestinal Epithelial Cells Following Vibrio cholerae Infection
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Transcriptional Upregulation of Inflammatory Cytokines in
Human Intestinal Epithelial Cells Following Vibrio cholerae
Infection
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| Creator |
Bandyopadhaya, Arunava
Sarkar, Madhubanti Chaudhuri, Keya |
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| Subject |
Molecular & Human Genetics
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| Description |
Coordinated expression and upregulation of interleukin-1a, interleukin-1b, tumor necrosis factor-a, interleukin-6, granulocyte–macrophage colonystimulating factor, interleukin-8, monocyte chemotactic protein-1 (MCP-1) and epithelial cell derived neutrophil activator-78, with chemoattractant and proinflammatory properties of various cytokine families, were obtained in the intestinal epithelial cell line Int407 upon Vibrio cholerae infection.
These proinflammatory cytokines also showed increased expression in T84 cells, except for interleukin-6, whereas a striking dissimilarity in cytokine expression was observed in Caco-2 cells. Gene expression studies of MCP-
1, granulocyte–macrophage colony-stimulating factor, interleukin-1a, interleukin- 6 and the anti-inflammatory cytokine transforming growth factor-b in Int407 cells with V. cholerae culture supernatant, cholera toxin, lipopolysaccharide and ctxA mutant demonstrated that, apart from cholera toxin and lipopolysaccharide, V. cholerae culture supernatant harbors strong inducer(s) of interleukin-6 and MCP-1 and moderate inducer(s) of interleukin- 1a and granulocyte–macrophage colony-stimulating factor. Cholera toxin- or lipopolysaccharide-induced cytokine expression is facilitated by
activation of nuclear factor-jB (p65 and p50) and cAMP response elementbinding protein in Int407 cells. Studies with ctxA mutants of V. cholerae revealed that the mutant activates the p65 subunit of nuclear factor-jB and
cAMP response element-binding protein, and as such the activation is mediated by cholera toxin-independent factors as well. We conclude that V. cholerae elicits a proinflammatory response in Int407 cells that is mediated
by activation of nuclear factor-jB and cAMP response element-binding protein by cholera toxin, lipopolysaccharide and ⁄ or other secreted products of V. cholerae.
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| Date |
2007
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| Type |
Article
PeerReviewed |
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| Relation |
http://dx.doi.org/10.1111/j.1742-4658.2007.05991.x
http://www.eprints.iicb.res.in/265/ |
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| Identifier |
Bandyopadhaya, Arunava and Sarkar, Madhubanti and Chaudhuri, Keya (2007) Transcriptional Upregulation of Inflammatory Cytokines in Human Intestinal Epithelial Cells Following Vibrio cholerae Infection. FEBS Journal, 274 (17). pp. 4631-4642.
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