Molecular Characterization of Vibrio Cholerae �relA �spoT Double Mutants
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
Molecular Characterization of Vibrio Cholerae �relA �spoT Double Mutants
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| Creator |
Das, Bhabatosh
Bhadra, Rupak K |
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| Subject |
Infectious Diseases and Immunology
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| Description |
In Escherichia coli cellular levels of pppGpp
and ppGpp, collectively called (p)ppGpp, are maintained
by the products of two genes, relA and spoT. Like E. coli,
Vibrio cholerae also possesses relA and spoT genes. Here
we show that similar to E. coli, V. cholerae �relA cells can accumulate (p)ppGpp upon carbon starvation but not under
amino acid starved condition. Although like in E. coli, the
spoT gene function was found to be essential in V. cholerae
relA+ background, but unlike E. coli, several V. cholerae
�relA �spoT mutants constructed in this study accumulated
(p)ppGpp under glucose starvation. The results suggest a
cryptic source of (p)ppGpp synthesis in V. cholerae, which
is induced upon glucose starvation. Again, unlike E. coli
�relA �spoT mutant (ppGpp0 strain), the V. cholerae �relA
�spoT mutants showed certain unusual phenotypes, which
are (a) resistance towards 3-amino-1,2,4-triazole (AT); (b)
growth in nutrient poor M9 minimal medium; (c) ability to
stringently regulate cellular rRNA accumulation under glucose starvation and (d) initial growth defect in nutrient rich medium. Since these phenotypes of �relA �spoT mutants could be reverted back to �relA phenotypes by providing SpoT in trans, it appears that the spoT gene function is crucial
in V. cholerae.
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| Date |
2008
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/287/1/ARCHIVES_OF_MICROBIOLOGY%2C189_(3)%2C_227%2D238%2C2008[118].pdf
Das, Bhabatosh and Bhadra, Rupak K (2008) Molecular Characterization of Vibrio Cholerae �relA �spoT Double Mutants. Arch Microbiol, 189 (3). pp. 227-238. |
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| Relation |
http://dx.doi.org/10.1007/s00203-007-0312-z
http://www.eprints.iicb.res.in/287/ |
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