DNA Repair Deficiency Leads to Susceptibility to Develop Arsenic-Induced Premalignant Skin Lesions
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
View Archive Info| Field | Value | |
| Title |
DNA Repair Deficiency Leads to Susceptibility to Develop Arsenic-Induced Premalignant Skin Lesions
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| Creator |
Banerjee, Mayukh
Sarma, Nilendu Biswas, Rupanwita Roy, Jyoeeta Mukherjee, Anita Giri, Ashok K |
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| Subject |
Molecular & Human Genetics
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| Description |
In West Bengal, India, although more than 6 million people are exposed to arsenic through drinking water, only 15–20% showed arsenic-induced skin lesions, including premalignant hyperkeratosis. This indicates toward some factors that confer susceptibility to arsenic-induced carcinogenicity. In this work, we wanted to explore whether differences in DNA repair capacity could impart arsenic-induced carcinogenicity, through Comet assay, chromosomal
aberration (CA) assay and challenge assay. Sixty arsenic
exposed (30 individuals with arsenic-induced premalignant hyperkeratosis and 30 without skin lesion, but drinking similar arsenic contaminated water) and 30 arsenic unexposed individuals were recruited as study participants. Alkaline comet assay, and challenge assay were carried out in whole blood and CA study in lymphocytes
to find out the DNA damage and DNA repair capacity in
both hyperkeratotic and without skin lesion individuals. DNA
damage as well as CA were found to be significantly higher in the arsenic-exposed individuals compared to unexposed individuals (p < 0.001). Within the exposed group, there was no significant difference as far as the level of DNA damage is concerned (p > 0.05), but CA was significantly higher in exposed individuals with hyperkeratosis
than exposed individuals without hyperkeratosis (p <
0.01). Challenge assay showed that upon induction of DNA damage, the repair capacity in the exposed individuals with premalignant hyperkeratosis is significantly less (p < 0.001) than that of individuals without skin lesion, although the basal level of DNA damage was similar in both. Thus, the deficiency in DNA repair capacities in the hyperkeratotic individuals emerges as a prime
contender for arsenic carcinogenicity.
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| Date |
2008
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/289/1/INTERNATIONAL_JOURNAL_OF_CANCER%2C_123(_2)%2C283%2D287%2C2008[51].pdf
Banerjee, Mayukh and Sarma, Nilendu and Biswas, Rupanwita and Roy, Jyoeeta and Mukherjee, Anita and Giri, Ashok K (2008) DNA Repair Deficiency Leads to Susceptibility to Develop Arsenic-Induced Premalignant Skin Lesions. Int. J. Cancer, 123 (2). pp. 283-287. |
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| Relation |
http://dx.doi.org/10.1002/ijc.23478
http://www.eprints.iicb.res.in/289/ |
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