Apoptotic Mode of Cell Death in Substantia Nigra Following Intranigral Infusion of the Parkinsonian Neurotoxin, MPP+ in Sprague-Dawley Rats: Cellular, Molecular and Ultrastructural Evidences
IR@IICB: CSIR-Indian Institute of Chemical Biology, Kolkata
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| Title |
Apoptotic Mode of Cell Death in Substantia Nigra Following
Intranigral Infusion of the Parkinsonian Neurotoxin, MPP+ in
Sprague-Dawley Rats: Cellular, Molecular and Ultrastructural
Evidences
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| Creator |
Banerjee, Rebecca
Sen, Sreetama Saravanan, Karuppagounder S Dey, Sailendra Nath Mohanakumar , K P |
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| Subject |
Cell Biology & Physiology
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| Description |
The potent parkinsonian neurotoxin 1-methyl-
4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP) is
known to cause dopaminergic neurodegeneration in
nigrostriatal system. In the present study we investigated
the nuclear morphology of cells in the substantia
nigra pars compacta (SNpc) region of
rats following unilateral intranigral infusion of the
active metabolite, 1-methyl-4-phenyl pyridinium ion
(MPP+), which resulted in a dose-dependent and
prolonged dopamine depletion in the ipsilateral striatum.
There appeared a substantial loss of tyrosine
hydroxylase immunoreactive neurons in the SNpc that
received the neurotoxin. Specific nuclear staining with
Hoechst 33342 or acridine orange revealed bright
pyknotic, shrunken, distorted nuclei and condensed
chromatin with perinuclear nucleolus respectively
following visualization with the former and latter dyes
in the ipsilateral SNpc, as compared to the round,
intact nuclei and centrally positioned nucleolus in the
contralateral side. Ultrastructural details of the nucleus
under transmission electron microscope confirmed
distorted nuclear organization with shrunken
or condensed nuclei and disrupted nuclear membrane.
These features are typical of nucleus undergoing
apoptosis, and suggest that MPP+ causes dopaminergic neuronal death through an apoptotic mode. Typical
laddering pattern of genomic DNA isolated from
the ipsilateral SN in agarose gel electrophoresis conclusively
established apoptosis following intranigral
administration of MPP+ in rats.
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| Date |
2007
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/310/1/NEUROCHEMICAL_RESEARCH%2C_32(_7)%2C__1238%2D1247[67].pdf
Banerjee, Rebecca and Sen, Sreetama and Saravanan, Karuppagounder S and Dey, Sailendra Nath and Mohanakumar , K P (2007) Apoptotic Mode of Cell Death in Substantia Nigra Following Intranigral Infusion of the Parkinsonian Neurotoxin, MPP+ in Sprague-Dawley Rats: Cellular, Molecular and Ultrastructural Evidences. Neurochem Research, 32 (7). pp. 1238-1247. |
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| Relation |
http://dx.doi.org/10.1007/s11064-007-9299-8
http://www.eprints.iicb.res.in/310/ |
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